Huff K K, Lippman M E
Endocrinology. 1984 May;114(5):1702-10. doi: 10.1210/endo-114-5-1702.
Activity of secreted plasminogen activator (PA) by ZR-75-1 human breast cancer cells in culture is shown to be altered by the addition of physiologically relevant concentrations of the hormones 17 beta-estradiol (E2), insulin, and dexamethasone. After 48 h, E2 stimulated PA activity 6-fold at concentrations as low as 10(-12) M. This stimulation was prevented by the addition of actinomycin D and cycloheximide. The antiestrogen tamoxifen reduced estrogen stimulation of PA, but had slight stimulatory effects on PA secretion by itself. Insulin (5 X 10(-10) M) induced a 2-fold increase in PA activity. Effects of insulin and E2 were additive, suggesting independent sites of control of PA production. Dexamethasone (10(-8) M) decreased PA activity by 20%, but did not inhibit cell growth at the concentration tested. These data suggest that secreted PA activity is differentially regulated by hormones and that effects of PA and growth do not occur in parallel.
培养中的ZR - 75 - 1人乳腺癌细胞分泌的纤溶酶原激活物（PA）的活性，被证明会因添加生理相关浓度的激素17β - 雌二醇（E2）、胰岛素和地塞米松而改变。48小时后，低至10(-12) M浓度的E2可将PA活性刺激6倍。添加放线菌素D和环己酰亚胺可阻止这种刺激。抗雌激素他莫昔芬可降低雌激素对PA的刺激，但自身对PA分泌有轻微刺激作用。胰岛素（5×10(-10) M）可使PA活性增加2倍。胰岛素和E2的作用是相加的，表明PA产生的控制位点是独立的。地塞米松（10(-8) M）可使PA活性降低20%，但在所测试的浓度下不抑制细胞生长。这些数据表明，分泌的PA活性受激素的差异调节，且PA和生长的作用并非平行发生。
