Lemaitre B, Coen D
Mécanismes moléculaires de la spéciation, Université Pierre et Marie Curie, Paris, France.
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4419-23. doi: 10.1073/pnas.88.10.4419.
The transposition of P elements in Drosophila melanogaster is regulated by products encoded by the P elements themselves. The molecular mechanisms of this regulation are complex and still unclear. We have assayed in vivo the effects of P regulatory products on the P promoter itself by using P-lacZ fusion genes. We have found that all the P-lacZ insertions are repressed in a P background. This repression occurs in all the tissues observed and at all the developmental stages. The amount of transcripts specific for P-lacZ is substantially reduced in a P background. These results suggest that P trans-acting products can exert a direct repression on the P promoter transcription.
黑腹果蝇中P因子的转座受P因子自身编码产物的调控。这种调控的分子机制很复杂，目前仍不清楚。我们通过使用P-lacZ融合基因在体内检测了P调控产物对P启动子本身的影响。我们发现，所有P-lacZ插入在P背景中都受到抑制。这种抑制在所有观察到的组织以及所有发育阶段都存在。在P背景中，P-lacZ特异性转录本的量大幅减少。这些结果表明，P反式作用产物可对P启动子转录施加直接抑制作用。
