Vaslet C A, O'Connell P, Izquierdo M, Rosbash M
Nature. 1980 Jun 26;285(5767):674-6. doi: 10.1038/285674a0.
Molecular cloning techniques are particularly well suited to the study of gene organization in Drasophila melanogaster because recombinant DNA can easily be localized in the genome by in situ hybridization to salivary gland polytene chromosomes. We report here the isolation and preliminary characterization of a recombinant phage, designated C25, containing a bona fide D. melanogaster ribosomal protein gene. In situ hybridization demonstrates that this sequence maps to region 99D on chromosome 3.
分子克隆技术特别适合于研究黑腹果蝇的基因组织,因为通过与唾液腺多线染色体进行原位杂交,重组DNA能够很容易地定位在基因组中。我们在此报告一种重组噬菌体(命名为C25)的分离及初步特性分析,该噬菌体含有一个真正的黑腹果蝇核糖体蛋白基因。原位杂交表明,此序列定位于3号染色体的99D区域。