Khlif M, Sellami H, Sellami A, Chelly H, Makni F, Bouaziz M, Ayadi A
Fungal and Parasitic Molecular Biology Laboratory, School of Medicine Sfax, Sfax, Tunisia.
Mycoses. 2009 Mar;52(2):171-5. doi: 10.1111/j.1439-0507.2008.01546.x. Epub 2008 Jun 3.
Candida dubliniensis, a newly described fungal pathogen associated mainly to immunocompromised host's infection, is phenotypically closely related to C. albicans. In this study, we report for the first time, isolation and identification, in Tunisia, of 14 isolates of C. dubliniensis from 12 human immunodeficiency virus-negative patients hospitalised in the intensive care unit (ICU) at Habib Bourguiba Sfax Hospital. Our study was firstly based on the failure to grow at 45 degrees C. This presumptive identification was completed by other tests: chlamydospore production, culture on Candiselect4 (Bio-Rad) and the commercial test Bichro-Dubli fumouze, which specifically identify C. dubliniensis. The confirmation of the discrimination between both species was performed by PCR, targeting the hyphal wall protein (HWP1) gene. The recovery of C. dublinensis by routine laboratory diagnosis is recommended for elucidating the epidemiology of this novel pathogen.
都柏林念珠菌是一种新发现的主要与免疫功能低下宿主感染相关的真菌病原体，在表型上与白色念珠菌密切相关。在本研究中，我们首次报告了在突尼斯从斯法克斯哈比卜·布尔吉巴医院重症监护病房（ICU）住院的12例人类免疫缺陷病毒阴性患者中分离并鉴定出14株都柏林念珠菌。我们的研究首先基于其在45摄氏度下无法生长。这种初步鉴定通过其他测试得以完善：厚垣孢子产生、在Candiselect4（伯乐公司）上培养以及商业测试Bichro-Dubli fumouze，后者可特异性鉴定都柏林念珠菌。通过针对菌丝壁蛋白（HWP1）基因的PCR对两种念珠菌之间的鉴别进行了确认。建议通过常规实验室诊断来分离都柏林念珠菌，以阐明这种新型病原体的流行病学情况。
