Ozkumur Emre, Needham James W, Bergstein David A, Gonzalez Rodrigo, Cabodi Mario, Gershoni Jonathan M, Goldberg Bennett B, Unlü M Selim
Department of Electrical and Computer Engineering, Boston University, Boston, MA 02215, USA.
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):7988-92. doi: 10.1073/pnas.0711421105. Epub 2008 Jun 3.
Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm(2) SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.
无需二级反应物即可直接监测初级分子结合相互作用，这将显著简化并扩展高通量无标记检测方法的应用。本文介绍了一种简单的干涉测量技术，该技术可监测由累积生物分子质量引起的光学相位差。例如，对由牛血清白蛋白、人血清白蛋白、兔免疫球蛋白和蛋白G组成的四种蛋白质中的每一种的50个斑点在捕获相应抗体时进行了动态监测。动态测量的标准差为每个斑点26 pg/mm²，可检测浓度为19 ng/ml。所提出的方法对于蛋白质微阵列分析特别适用，因为它无标记、简单、灵敏且易于扩展至高通量。
