Department of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14526, USA.
Department of Dermatology, University of Rochester, Rochester, NY 14526, USA.
Biosensors (Basel). 2023 Sep 5;13(9):870. doi: 10.3390/bios13090870.
Arrayed imaging reflectometry (AIR), first introduced in 2004, is a thin-film interference sensor technique that optimizes optical properties (angle of incidence, polarization, substrate refractive index, and thickness) to create a condition of total destructive interference at the surface of a silicon substrate. The advantages of AIR are its sensitivity, dynamic range, multiplex capability, and high-throughput compatibility. AIR has been used for the detection of antibodies against coronaviruses, influenza viruses, , and human autoantigens. It has also shown utility in detection of cytokines, with sensitivity comparable to bead-based and ELISA assays. Not limited to antibodies or antigens, mixed aptamer and protein arrays as well as glycan arrays have been employed in AIR for differentiating influenza strains. Mixed arrays using direct and competitive inhibition assays have enabled simultaneous measurement of cytokines and small molecules. Finally, AIR has also been used to measure affinity constants, kinetic and at equilibrium. In this review, we give an overview of AIR biosensing technologies and present the latest AIR advances.
列阵成像反射率测量技术（Arrayed Imaging Reflectometry，AIR）于 2004 年首次问世，是一种薄膜干涉传感器技术，它优化了光学特性（入射角、偏振、基底折射率和厚度），在硅基底表面创造了完全破坏性干涉的条件。AIR 的优点在于其灵敏度、动态范围、多路复用能力和高通量兼容性。AIR 已被用于检测冠状病毒、流感病毒、和人类自身抗原的抗体。它在细胞因子的检测中也显示出了实用性，其灵敏度可与基于珠粒和 ELISA 的检测相媲美。不仅限于抗体或抗原，混合适体和蛋白质阵列以及聚糖阵列也已被用于 AIR 中以区分流感株。使用直接和竞争抑制测定的混合阵列使得同时测量细胞因子和小分子成为可能。最后，AIR 也被用于测量亲和力常数、动力学和平衡。在这篇综述中，我们概述了 AIR 生物传感技术，并介绍了最新的 AIR 进展。
