Rasouli Manoochehr, Kiany Simin, Behbin Maryam
Department of Immunology, Prof. Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz, Iran.
Iran J Immunol. 2008 Jun;5(2):131-5.
Interleukin-10 (IL-10) is a Th2-type cytokine that inhibits macrophage activation. It is known that production of IL-10 is affected by its gene promoter polymorphisms.
To investigate the relationship between IL-10 gene promoter polymorphisms and susceptibility to brucellosis.
One hundred and ninety patients with brucellosis and 81 healthy animal husbandmen who owned infected animals and consumed their contaminated dairy products were included in this study. All individuals were genotyped for three bi-allelic IL-10 gene promoter polymorphisms at positions -1082(G/A), -819(T/C), and -592(A/C) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The distribution of C alleles at positions -592 and -819 of IL-10 were significantly higher in patients than in the healthy animal husbandmen (p=0.034 and p=0.0086, respectively). IL-10 ATA single and double haplotypes were significantly higher in controls, compared to the patients (p= 0.0278 and p=0.013, respectively).
According to the results higher frequency of C alleles at positions -592 and -819 of IL-10 in patients may be considered as genetic factors for susceptibility to brucellosis.
白细胞介素-10（IL-10）是一种抑制巨噬细胞活化的Th2型细胞因子。已知IL-10的产生受其基因启动子多态性的影响。
探讨IL-10基因启动子多态性与布鲁氏菌病易感性之间的关系。
本研究纳入了190例布鲁氏菌病患者和81名拥有感染动物并食用其受污染乳制品的健康牧民。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对所有个体的IL-10基因启动子-1082（G/A）、-819（T/C）和-592（A/C）位点的三个双等位基因多态性进行基因分型。
患者中IL-10基因-592和-819位点C等位基因的分布显著高于健康牧民（分别为p = 0.034和p = 0.0086）。与患者相比，对照组中IL-10 ATA单倍型和双倍型的比例显著更高（分别为p = 0.0278和p = 0.013）。
根据研究结果，患者中IL-10基因-592和-819位点C等位基因的高频率可能被视为布鲁氏菌病易感性的遗传因素。
