Budak Ferah, Göral Güher, Heper Yasemin, Yilmaz Emel, Aymak Figen, Baştürk Bilkay, Töre Okan, Ener Beyza, Oral Haluk B
Microbiology and Infectious Diseases Department, Faculty of Medicine, Uludağ University, 16059 Bursa, Turkey.
Cytokine. 2007 Apr;38(1):32-6. doi: 10.1016/j.cyto.2007.04.008. Epub 2007 Jun 4.
Several genes encoding for different cytokines may play crucial roles in host susceptibility to Brucellosis, since the cytokine production capacity varies among individuals and depends on the cytokine gene polymorphism. The association of the cytokine gene polymorphisms with the development of Brucellosis was investigated in this study. DNA samples were obtained from a Turkish population of 40 patients with Brucellosis, and 50 healthy control subjects. All genotyping (IL-6, IL10, IFN-gamma, TGF-beta and TNF-alpha) experiments were performed using sequence-specific primers PCR (PCR-SSP). When compared to the healthy controls, the frequencies of high/intermediate producing genotypes of IL-10 and high producing genotype of IL-6 were significantly more common in the patient group. These results suggest that IL-10 and IL-6 gene polymorphisms may affect susceptibility to Brucellosis and increase risk of developing the disease. In order to confirm the biological significance of our results, further studies should be performed in larger population groups.
由于个体间细胞因子产生能力存在差异且取决于细胞因子基因多态性，因此编码不同细胞因子的几个基因可能在宿主对布鲁氏菌病的易感性中发挥关键作用。本研究调查了细胞因子基因多态性与布鲁氏菌病发病的关联。从土耳其的40例布鲁氏菌病患者和50例健康对照者中获取DNA样本。所有基因分型（IL-6、IL10、IFN-γ、TGF-β和TNF-α）实验均使用序列特异性引物PCR（PCR-SSP）进行。与健康对照相比，患者组中IL-10高/中等产生基因型和IL-6高产生基因型的频率明显更高。这些结果表明，IL-10和IL-6基因多态性可能影响对布鲁氏菌病的易感性并增加患病风险。为了证实我们结果的生物学意义，应在更大的人群中进行进一步研究。
