Abu-Elamreen Farid H, Abed Abdalla A, Sharif Fadel A
Department of Medical Microbiology, Central Laboratory and Blood Bank, AlShifa Hospital, Palestinian Ministry of Health, Gaza, Palestine.
Med Princ Pract. 2008;17(4):296-301. doi: 10.1159/000129609. Epub 2008 Jun 3.
To determine the etiology of acute diarrhea in Palestinian children under 5 years of age and to improve knowledge of the etiology of gastrointestinal pathogens using traditional and molecular diagnostic techniques.
Various common enteropathogens (viral, bacterial and parasites) associated with diarrhea were investigated by conventional and molecular techniques (PCR) in 150 children less than 5 years of age admitted to the Central Pediatric Hospital, Gaza Strip, Palestine.
The occurrence of enteropathogens identified was as follows: rotavirus 42/150 (28%), Entamoeba histolytica/dispar 23/150 (15%), Shigella spp. 9/150 (6%), Campylobacter coli/jejuni and Escherichia coli O157:H7 7/150 (5%) each, Salmonella spp. 3/150 (2%), Giardia intestinalis 1/150 (1%), and Strongyloides stercoralis 1/150 (1%) of the samples. Shigella and Salmonella isolates were tested for their susceptibility to common antimicrobial agents and most of the isolates were resistant to ampicillin and trimethoprim/sulfamethoxazole.
This study demonstrated that rotavirus, E. coli O157:H7 and Campylobacter, which are not routinely screened for in Gaza Strip, were significant enteropathogens. The results highlight the value of using a combination of traditional and PCR techniques in the diagnosis of enteropathogens related to gastroenteritis.
确定巴勒斯坦5岁以下儿童急性腹泻的病因，并利用传统和分子诊断技术提高对胃肠道病原体病因的认识。
采用传统和分子技术（聚合酶链反应）对巴勒斯坦加沙地带中央儿童医院收治的150名5岁以下儿童中与腹泻相关的各种常见肠道病原体（病毒、细菌和寄生虫）进行调查。
已鉴定出的肠道病原体发生率如下：轮状病毒42/150（28%），溶组织内阿米巴/迪斯帕内阿米巴23/150（15%），志贺菌属9/150（6%），空肠弯曲菌/结肠弯曲菌和大肠杆菌O157:H7各7/150（5%），沙门菌属3/150（2%），蓝氏贾第鞭毛虫1/150（1%），粪类圆线虫1/150（1%）。对志贺菌和沙门菌分离株进行了对常见抗菌药物的敏感性测试，大多数分离株对氨苄西林和甲氧苄啶/磺胺甲恶唑耐药。
本研究表明，在加沙地带未进行常规筛查的轮状病毒、大肠杆菌O157:H7和弯曲菌是重要的肠道病原体。结果突出了联合使用传统技术和聚合酶链反应技术诊断与肠胃炎相关肠道病原体的价值。
