Abiaka Clifford, Al-Tobi Mohammed, Joshi Ravi
Department of Biochemistry, College of Medicine and Health Sciences, Sultan Qaboos University, Al-Khoudh, Oman.
Med Princ Pract. 2008;17(4):334-9. doi: 10.1159/000129616. Epub 2008 Jun 3.
This study aimed to establish reference ranges of serum concentrations of copper, zinc, retinol, alpha-tocopherol, copper:caeruloplasmin and copper:zinc ratios in a group of healthy Omani men and women.
Assay techniques employed were atomic absorption spectrophotometry (copper and zinc), reverse-phase high-pressure liquid chromatography with isocratic elution (retinol and alpha-tocopherol), immunonephelometry (caeruloplasmin) and spectrophotometry (albumin and cholesterol).
The mean +/- SD (microM) obtained for copper, zinc, retinol, and alpha-tocopherol were 15.9 +/- 3.0, 14.2 +/- 2.0, 1.45 +/- 0.39 and 16.9 +/- 4.4, respectively. The mean +/- SD for copper:zinc and copper:caeruloplasmin ratios were 1.15 +/- 0.30 micromol/mmol and 6.99 +/- 0.84 micromol/g, respectively. Significantly higher (p < 0.0001) copper and caeruloplasmin concentrations, copper:zinc and copper:caeruloplasmin ratios and lower zinc, retinol, alpha-tocopherol, cholesterol concentrations and alpha-tocopherol:cholesterol ratio were present in women compared to men. Age appeared to be associated with copper and retinol concentrations, and copper:caeruloplasmin ratios in women; in men, the association was mostly moderate with caeruloplasmin, alpha-tocopherol, cholesterol concentrations and alpha-tocopherol:cholesterol ratios. Smokers had decreased albumin (p = 0.002), zinc (p = 0.023) concentrations, and copper:caeruloplasmin ratios (p = 0.002), increased alpha-tocopherol concentrations (p = 0.016) and alpha-tocopherol:cholesterol ratios (p = 0.021) compared with non-smokers. Deficiency incidence was < or =5% for all investigated parameters.
Reference ranges of micronutrient concentrations and micromineral ratios were established for Omani subjects. The mean values of several micronutrients were lower than those reported for other populations and some showed gender effects.
本研究旨在确定一组健康阿曼男性和女性血清铜、锌、视黄醇、α-生育酚、铜：铜蓝蛋白和铜：锌比值的参考范围。
所采用的检测技术包括原子吸收分光光度法（检测铜和锌）、等度洗脱反相高效液相色谱法（检测视黄醇和α-生育酚）、免疫比浊法（检测铜蓝蛋白）以及分光光度法（检测白蛋白和胆固醇）。
铜、锌、视黄醇和α-生育酚的平均±标准差（μM）分别为15.9±3.0、14.2±2.0、1.45±0.39和16.9±4.4。铜：锌和铜：铜蓝蛋白比值的平均±标准差分别为1.15±0.30 μmol/mmol和6.99±0.84 μmol/g。与男性相比，女性的铜和铜蓝蛋白浓度、铜：锌和铜：铜蓝蛋白比值显著更高（p<0.0001），而锌、视黄醇、α-生育酚、胆固醇浓度以及α-生育酚：胆固醇比值更低。年龄似乎与女性的铜和视黄醇浓度以及铜：铜蓝蛋白比值相关；在男性中，这种关联大多与铜蓝蛋白、α-生育酚、胆固醇浓度以及α-生育酚：胆固醇比值呈中等程度相关。与不吸烟者相比，吸烟者的白蛋白（p = 0.002）、锌（p = 0.023）浓度以及铜：铜蓝蛋白比值（p = 0.002）降低，α-生育酚浓度（p = 0.016）和α-生育酚：胆固醇比值（p = 0.021）升高。所有研究参数的缺乏发生率均≤5%。
确定了阿曼受试者微量营养素浓度和微量矿物质比值的参考范围。几种微量营养素的平均值低于其他人群报告的值，并且一些显示出性别效应。
