Larralde-Corona C P, Santiago-Mena M R, Sifuentes-Rincón A M, Rodríguez-Luna I C, Rodríguez-Pérez M A, Shirai K, Narváez-Zapata J A
Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Blvd Del Maestro esq. Elias Piña, Reynosa, Tamaulipas, Mexico.
Appl Microbiol Biotechnol. 2008 Aug;80(1):167-77. doi: 10.1007/s00253-008-1532-0. Epub 2008 Jun 4.
Native strains of Trichoderma isolated from sorghum and common bean crop soils were investigated to assess their biocontrol potential over the phytopathogenic fungus Macrophomina phaseolina, isolated from diseased plants. The Trichoderma strains were characterized with a polyphasic approach, which combined the analysis of their morphological characteristics, enzymatic activity, macro- and microculture test results, rDNA restriction patterns (AFLP), ITS1-5.8S-ITS2 rDNA sequences, and protein profiles. The integration of these data sets can be used to select new isolates as biological control agents against native fungal phytopathogens. In general, we observed a positive correlation between the secretion of beta-1,3-glucanase and N-acetylhexosaminidase, and the biocontrol capacities of all the Trichoderma isolates. Strains with the best hyperparasitic behavior against M. phaseolina isolated from diseased bean and sorghum were Trichoderma sp. (TCBG-2) and Trichoderma koningiopsis (TCBG-8), respectively.
对从高粱和菜豆作物土壤中分离出的木霉本地菌株进行了研究，以评估它们对从患病植物中分离出的植物病原真菌菜豆壳球孢菌的生物防治潜力。采用多相方法对木霉菌株进行了表征，该方法结合了对其形态特征、酶活性、宏观和微观培养测试结果、rDNA限制性图谱（AFLP）、ITS1-5.8S-ITS2 rDNA序列和蛋白质谱的分析。这些数据集的整合可用于选择新的分离株作为对抗本地真菌植物病原体的生物防治剂。总体而言，我们观察到β-1,3-葡聚糖酶和N-乙酰己糖胺酶的分泌与所有木霉分离株的生物防治能力之间存在正相关。从患病菜豆和高粱中分离出的对菜豆壳球孢菌具有最佳重寄生行为的菌株分别是木霉菌（TCBG-2）和康宁木霉（TCBG-8）。
