Li Yaqian, Sun Ruiyan, Yu Jia, Saravanakumar Kandasamy, Chen Jie
Department of Environment and Resource, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai, 200240 People's Republic of China ; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, People's Republic of China ; Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, People's Republic of China.
Indian J Microbiol. 2016 Sep;56(3):318-27. doi: 10.1007/s12088-016-0581-9. Epub 2016 Apr 18.
The efficacy of seven strains of Trichoderma asperellum collected from the fields in Southern China was assessed against Fusarium graminearum (FG) the causal agent of corn stalk rot of maize were in vitro for their antagonistic properties followed by statistical model of principal compound analysis to identify the beneficial antagonist T. asperellum strain. The key factors of antagonist activity were attributed to a total of 13 factors including cell wall degrading enzymes (chitnase, protease and β-glucanases), secondary metabolites and peptaibols and these were analyzed from eight strains of Trichoderma. A linear regression model demonstrated that interaction of enzymes and secondary metabolites of T. asperellum strain ZJSX5003 enhanced the antagonist activity against FG. Further, this strain displayed a disease reduction of 71 % in maize plants inoculated with FG compared to negative control. Pointing out that the T. asperellum strain ZJSX5003 is a potential source for the development of a biocontrol agent against corn stalk rot.
对从中国南方田间采集的7株棘孢木霉菌株针对玉米茎腐病的病原菌禾谷镰刀菌（FG）进行了体外拮抗特性评估，随后采用主成分分析统计模型来鉴定有益的拮抗棘孢木霉菌株。拮抗活性的关键因素共有13个，包括细胞壁降解酶（几丁质酶、蛋白酶和β-葡聚糖酶）、次生代谢产物和短肽菌素，这些因素来自8株木霉菌株并进行了分析。线性回归模型表明，棘孢木霉菌株ZJSX5003的酶与次生代谢产物之间的相互作用增强了对FG的拮抗活性。此外，与阴性对照相比，该菌株对接种FG的玉米植株的病害减轻了71%。指出棘孢木霉菌株ZJSX5003是开发防治玉米茎腐病生物防治剂的潜在来源。
