Yoshii Toshitaka, Sotome Shinichi, Torigoe Ichiro, Tsuchiya Akio, Maehara Hidetsugu, Ichinose Shizuko, Shinomiya Kenichi
Section of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
J Orthop Res. 2009 Jan;27(1):1-7. doi: 10.1002/jor.20630.
Recent advances in tissue engineering techniques have allowed porous biomaterials to be combined with osteogenic cells for effective bone regeneration. We developed a simple low-pressure cell-loading method using only syringes and stopcocks, and examined the effect of this method on osteogenesis when applied to the combination of highly porous beta-tricalcium phosphate (beta-TCP) and fresh autologous bone marrow. Both block and granule beta-TCP scaffolds were used to prepare implants in three different ways: without bone marrow as a control, with bone marrow that was allowed to penetrate spontaneously under atmospheric pressure (AP group), and with bone marrow that was seeded under low pressure (ULP group). These implants were transplanted into rabbit intramuscular sites, and the samples were examined biologically and histologically. The penetration efficiency of the block implants after marrow introduction was significantly higher in the ULP group than in the AP group. In the transplanted block samples, alkaline phosphatase activity was significantly higher in the ULP group at 2 weeks after implantation, and significantly more newly formed bone was observed in the ULP group at both 5 and 10 weeks compared with the AP group. Similar results were observed even in the experiment using beta-TCP granules, which are smaller than the blocks and frequently used clinically. Because of its convenience and safety, this low-pressure method might be a novel, effective treatment to promote osteogenesis with bone marrow in clinical bone reconstruction surgeries.
组织工程技术的最新进展使得多孔生物材料能够与成骨细胞结合，以实现有效的骨再生。我们开发了一种仅使用注射器和旋塞的简单低压细胞加载方法，并研究了该方法应用于高度多孔的β-磷酸三钙（β-TCP）与新鲜自体骨髓组合时对骨生成的影响。块状和颗粒状β-TCP支架均用于以三种不同方式制备植入物：无骨髓作为对照，有在大气压下自发渗透的骨髓（AP组），以及有在低压下接种的骨髓（ULP组）。将这些植入物移植到兔肌肉内部位，并对样本进行生物学和组织学检查。骨髓引入后，ULP组块状植入物的渗透效率显著高于AP组。在移植的块状样本中，植入后2周时ULP组的碱性磷酸酶活性显著更高，与AP组相比，在5周和10周时ULP组观察到的新形成骨明显更多。即使在使用比块状更小且临床上常用的β-TCP颗粒的实验中也观察到了类似结果。由于其便利性和安全性，这种低压方法可能是临床骨重建手术中促进骨髓骨生成的一种新颖、有效的治疗方法。
