Kim Dong-Woo, Hwang In Koo, Kim Dae Won, Yoo Ki-Yeon, Won Chung-Kil, Moon Won-Kook, Won Moo-Ho
Research Center, Natural F&P Co., Ltd, Chuncheon, South Korea.
Biofactors. 2007;30(3):139-47. doi: 10.1002/biof.5520300301.
Coenzyme Q_{10} (CoQ_{10}) is a naturally occurring antioxidant and a prominent component of mitochondrial electron transport chain. In the present study, we investigated the effect of CoQ_{10} nanoparticle against photoaging using immunohistochemistry and Western blot analysis in the hairless mouse skin induced by ultraviolet B (UVB) irradiation (300 mJ/cm;{2}, 3 min/day for 21 days). In the UVB-irradiated distilled water (DW)-treated group, manganese superoxide dismutase (SOD2) and glutathione peroxidase (GPx) immunoreactivity and their protein levels in the skin were significantly lower than those in the control group. However, SOD2 and GPx immunoreactivity and their protein levels in the skin of the UVB-irradiated CoQ_{10}-treated group were higher than those in the UVB-irradiated DW-treated group. GPx activity in the skin in the UVB-irradiated DW-treated group significantly decreased compared to that in the control group; whereas GPx activity in the UVB-irradiated CoQ_{10}-treated group was similar to that in the control group. These results suggest that CoQ_{10} strongly inhibits oxidative stress in the skin induced by UVB via increasing SOD2 and GPx.
辅酶Q10（CoQ10）是一种天然存在的抗氧化剂，也是线粒体电子传递链的重要组成部分。在本研究中，我们采用免疫组织化学和蛋白质印迹分析方法，研究了CoQ10纳米颗粒对紫外线B（UVB）照射（300 mJ/cm²，每天3分钟，共21天）诱导的无毛小鼠皮肤光老化的影响。在UVB照射的蒸馏水（DW）处理组中，皮肤中锰超氧化物歧化酶（SOD2）和谷胱甘肽过氧化物酶（GPx）的免疫反应性及其蛋白水平显著低于对照组。然而，UVB照射的CoQ10处理组皮肤中SOD2和GPx的免疫反应性及其蛋白水平高于UVB照射的DW处理组。与对照组相比，UVB照射的DW处理组皮肤中的GPx活性显著降低；而UVB照射的CoQ10处理组中的GPx活性与对照组相似。这些结果表明，CoQ10通过增加SOD2和GPx来强烈抑制UVB诱导的皮肤氧化应激。
