Ferreira Fernanda Oliveira, Ribeiro Fernanda Luiza Leite, Batista Aline Carvalho, Leles Cláudio Rodrigues, de Cássia Gonçalves Alencar Rita, Silva Tarcília Aparecida
Department of Oral Surgery and Pathology, Dental School, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Tumour Biol. 2008;29(2):114-21. doi: 10.1159/000137669. Epub 2008 Jun 5.
AIMS/METHODS: We investigated the expression of CCL2 by immunohistochemistry in samples of oral cavity squamous cell carcinoma (OCSCC), lip squamous cell carcinoma (LSCC) and metastatic and nonmetastatic lymph nodes. The relationship of CCL2 with survival data was also evaluated.
The percentage of CCL2+ cells in tumor parenchyma was similar in OCSCC and LSCC. In stroma, OCSCC showed a higher number of CCL2+ cells when compared with LSCC. In contrast, higher CD68+ macrophage counts were observed in LSCC. Low macrophage counts were significantly related to a greater proliferative index in tumoral cells. The density of CCL2+ nodal cells was significantly higher in metastatic lymph nodes when compared with nonmetastatic lymph nodes. When considering CCL2 in the parenchyma, the mean survival time for the patients with high CCL2+ cell counts was lower than that for patients with low CCL2+ cell counts. In contrast, in stroma, the mean survival time for the patients with high CCL2+ cell percentages was higher than for those with low CCL2+ cell percentages.
Our findings suggest a role for CCL2 in the spreading of tumoral cells to the lymph node in OCSCC. Furthermore, the CCL2-independent macrophage infiltration may play a role in determining less aggressive behavior.
目的/方法:我们通过免疫组织化学法研究了口腔鳞状细胞癌(OCSCC)、唇鳞状细胞癌(LSCC)以及转移和非转移淋巴结样本中CCL2的表达情况。还评估了CCL2与生存数据之间的关系。
OCSCC和LSCC肿瘤实质中CCL2+细胞的百分比相似。在间质中,与LSCC相比,OCSCC的CCL2+细胞数量更多。相反,在LSCC中观察到更高的CD68+巨噬细胞计数。低巨噬细胞计数与肿瘤细胞中更高的增殖指数显著相关。与非转移淋巴结相比,转移淋巴结中CCL2+淋巴结细胞的密度显著更高。当考虑实质中的CCL2时,CCL2+细胞计数高的患者的平均生存时间低于CCL2+细胞计数低的患者。相反,在间质中,CCL2+细胞百分比高的患者的平均生存时间高于CCL2+细胞百分比低的患者。
我们的研究结果表明CCL2在OCSCC肿瘤细胞向淋巴结扩散中起作用。此外,不依赖CCL2的巨噬细胞浸润可能在决定侵袭性较低的行为中起作用。