证实Fascin过表达是口腔鳞状细胞癌肿瘤微环境中肿瘤侵袭和淋巴结转移的关键调控因子。

Establishing Fascin over-expression as a strategic regulator of neoplastic aggression and lymph node metastasis in oral squamous cell carcinoma tumor microenvironment.

作者信息

Routray Samapika, Kheur Supriya, Chougule Hemlata M, Mohanty Neeta, Dash Rupesh

机构信息

Department of Oral Pathology and Microbiology, Institute of Dental Sciences, Siksha 'O'Anusandhan University, Bhubaneswar 751030, India; Department of Dental Surgery, All India Institute of Medical Sciences, Bhubaneswar-751019.

Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College & Hospital, D. Y. Patil Vidyapeeth, Pimri, Pune 411081, India.

出版信息

Ann Diagn Pathol. 2017 Oct;30:36-41. doi: 10.1016/j.anndiagpath.2017.05.013. Epub 2017 May 26.

DOI:10.1016/j.anndiagpath.2017.05.013

PMID:28965626

Abstract

INTRODUCTION

Oral squamous cell carcinoma (OSCC) has an aggressive behaviour with high incidence of nodal metastasis, even in the early stages, leading to poor prognosis. For progression and metastasis of cancers, the dominant element considered is cell motility. Fascin, an actin-binding protein has emerged as a protein of general importance for a diverse set of cell protrusions with functions in cell adhesion, cell interactions, and cell migration. The role of Fascin in various carcinomas, including aggressive behaviour in OSCC has been documented, but its role as a key regulator in lymph nodes metastasis is yet to be validated.

AIM

This study was piloted to evaluate and correlate Fascin expression in OSCC lymph nodes and understand the role of Fascin in contemptuous Lesional tissue, as a predictor of survival. A retrospective study designed with 40 archival OSCC cases was included as sample, 20 each of both lymph node metastasis +ve (Group 1) and -ve (Group 2) groups. All the participants were smokeless tobacco user and had tumor located at gingivo-buccal complex.

RESULTS

We established that Fascin over-expression in lymph nodes were significantly associated with clinico-histopathological parameters like staging (p=0.01), tumor size (cT) (p=0.03) and differentiation; and furthermore it was highly significant in correlation to nodal status (cN) (*p≤0.001). Fascin over-expression in lymph node metastasis positive cases correlated with that of Fascin expression in contemptuous Lesional tissue signifying its role in promoting aggressive progression and metastasis. This association was found to be statistically significant (p value=0.05). Overall Survival Analysis of both lymph node metastasis +ve and -ve groups assessed by Kaplan-Meier analysis (taking death and recurrence into consideration) showed patients with high Fascin expression (in lymph node and Lesional tissue) had shorter overall survival than patients who had no to weak Fascin expression.

CONCLUSION

Our findings thereby establish Fascin expression as a regulator of metastasis in OSCC tumor microenvironment and predictor of survival.

摘要

引言

口腔鳞状细胞癌（OSCC）具有侵袭性，即使在早期阶段，淋巴结转移发生率也很高，导致预后不良。对于癌症的进展和转移，细胞运动性被认为是主要因素。Fascin是一种肌动蛋白结合蛋白，已成为一种对多种细胞突起具有普遍重要性的蛋白质，在细胞黏附、细胞相互作用和细胞迁移中发挥作用。Fascin在各种癌症中的作用，包括在OSCC中的侵袭性行为，已有文献记载，但其作为淋巴结转移关键调节因子的作用尚未得到证实。

目的

本研究旨在评估并关联OSCC淋巴结中Fascin的表达，了解Fascin在相应病变组织中的作用，作为生存的预测指标。一项回顾性研究纳入了40例存档的OSCC病例作为样本，淋巴结转移阳性（第1组）和阴性（第2组）各20例。所有参与者均为无烟烟草使用者，肿瘤位于牙龈颊复合体。

结果

我们发现，淋巴结中Fascin的过表达与临床组织病理学参数如分期（p = 0.01）、肿瘤大小（cT）（p = 0.03）和分化显著相关；此外，它与淋巴结状态（cN）高度相关（*p≤0.001）。淋巴结转移阳性病例中Fascin的过表达与相应病变组织中Fascin的表达相关，表明其在促进侵袭性进展和转移中的作用。这种关联具有统计学意义（p值 = 0.05）。通过Kaplan-Meier分析（考虑死亡和复发）对淋巴结转移阳性和阴性两组进行的总生存分析表明，Fascin高表达（在淋巴结和病变组织中）的患者总生存期短于Fascin无表达至弱表达的患者。