Servoli Eva, Maniglio Devid, Motta Antonella, Migliaresi Claudio
Department of Materials Engineering and Industrial Technologies and INSTM Research Unit, University of Trento, via Mesiano 77, 38100 Trento, Italy.
Macromol Biosci. 2008 Sep 9;8(9):827-35. doi: 10.1002/mabi.200800057.
Silk fibroin from Bombyx mori is a high-molecular-weight protein, largely employed in the biomaterials field. Several parameters can affect the folding and assembly of fibroin heavy and light chains. The present work has shown that anisotropic and water-stable films are produced when fibroin solution is cast under an alternating electric field (AC). The treatment can affect the mechanical, thermal and surface properties of fibroin films. These effects have been related to the alignment of molecular dipoles and the formation of oriented supramolecular assemblies. Cell response is affected by this novel processing: MRC5 fibroblasts, cultured on anisotropic fibroin films, preferentially spread parallel to the field direction 6 h after seeding. [Figure: see text].
家蚕丝素蛋白是一种高分子量蛋白质，在生物材料领域有广泛应用。几个参数会影响丝素蛋白重链和轻链的折叠与组装。目前的研究表明，当丝素蛋白溶液在交变电场（AC）下浇铸时，会产生各向异性且水稳定的薄膜。这种处理会影响丝素蛋白薄膜的机械、热学和表面性能。这些影响与分子偶极子的排列以及定向超分子聚集体的形成有关。这种新型加工方法会影响细胞反应：接种6小时后，在各向异性丝素蛋白薄膜上培养的MRC5成纤维细胞优先沿电场方向平行铺展。[图：见正文]
