Kale Ramesh R, Mukundan Harshini, Price Dominique N, Harris J Foster, Lewallen Daniel M, Swanson Basil I, Schmidt Jurgen G, Iyer Suri S
Chemical and Biosensors group, 805 Crosley, Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221, USA.
J Am Chem Soc. 2008 Jul 2;130(26):8169-71. doi: 10.1021/ja800842v. Epub 2008 Jun 5.
We report the modular synthesis of robust, biotinylated biantennary sialylglycoconjugates and their ability to differentiate between two type A influenza strains. This is the first demonstration of glycoconjugate-based discriminatory capture and detection of two strains of intact influenza virus, in the presence of the innate enzymatic activity of viral neuraminidases. We also demonstrate a "carboassay" using glycoconjugates as capture and reporter elements, which therefore, does not require antibodies. The capture of intact influenza viruses is of potential benefit for clinical diagnostics.
我们报道了稳健的、生物素化的双天线唾液酸化糖缀合物的模块化合成及其区分两种甲型流感病毒株的能力。这是首次证明在病毒神经氨酸酶的固有酶活性存在的情况下，基于糖缀合物对两种完整流感病毒株进行鉴别捕获和检测。我们还展示了一种使用糖缀合物作为捕获和报告元件的“碳水化合物分析”方法，因此不需要抗体。完整流感病毒的捕获对临床诊断具有潜在益处。
