Slivka Peter F, Wong Johnny, Caputo Gregory A, Yin Hang
Department of Chemistry and Biochemistry, 215 UCB, University of Colorado, Boulder, Colorado 80309-0215, USA.
ACS Chem Biol. 2008 Jul 18;3(7):402-11. doi: 10.1021/cb800049w.
Much current interest in chemical biology focuses on the transmembrane domains of proteins, which have emerged as targets for the development of novel diagnostics and therapeutics. Integral membrane proteins are a group of important biomolecules that play pivotal roles in many cellular activities. Previous studies primarily focused on the extra- and/or intracellular domains of membrane proteins. However, the importance of transmembrane regions in the regulation of protein complexes is beginning to emerge. As such, a number of methods for designing and testing novel exogenous peptides that recognize transmembrane targets and modulate cellular functions have been developed. This Review outlines current methodologies for developing these transmembrane probes that may provide useful tools to study a variety of biological phenomena in the membrane.
当前，化学生物学的许多研究兴趣集中在蛋白质的跨膜结构域，这些跨膜结构域已成为新型诊断和治疗方法开发的靶点。整合膜蛋白是一类重要的生物分子，在许多细胞活动中发挥着关键作用。以往的研究主要集中在膜蛋白的胞外和/或胞内结构域。然而，跨膜区域在蛋白质复合物调控中的重要性正逐渐显现出来。因此，已经开发出了多种设计和测试新型外源性肽的方法，这些肽能够识别跨膜靶点并调节细胞功能。本综述概述了当前开发这些跨膜探针的方法，这些方法可能为研究膜内的各种生物学现象提供有用的工具。
