Toker Hulya, Ozan Fatih, Ozer Hatice, Ozdemir Hakan, Eren Kaya, Yeler Hasan
Department of Periodontology, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey.
J Periodontol. 2008 Jun;79(6):1089-94. doi: 10.1902/jop.2008.070462.
Propolis collected by honeybees from various plant sources is a resinous hive product possessing a broad spectrum of biologic activities. Propolis has been used extensively in the diet to improve health and prevent disease. The purpose of this study was to analyze the morphometric and histopathologic changes associated with experimental periodontitis in rats in response to the systemic administration of propolis.
Forty Wistar rats were divided into four experimental groups: non-ligated (NL; N = 10); ligature only (LO; N = 10); and systemic administration of ligature and propolis (100 mg/kg body weight per day [Pro100; N = 10] or 200 mg/kg body weight per day [Pro200; N = 10]). Silk ligatures were placed at the gingival margin of the lower first molars in both mandibular quadrants. The study duration was 11 days, and the animals were sacrificed at the end of this period. Changes in alveolar bone levels were clinically measured, and tissues were histopathologically examined to assess the differences among the study groups.
At the end of 11 days, alveolar bone loss was significantly higher in the LO group compared to the NL, Pro100, and Pro200 groups (P <0.05). Osteoclast numbers in the LO group were significantly higher than those of the NL, Pro100, and Pro200 groups (P <0.05). Both dosages of propolis significantly reduced the periodontitis-related bone loss, but the differences between the two propolis groups were not statistically significant (P >0.05).
The findings of this study provide morphologic and histologic evidence that propolis, when administered systemically, prevents alveolar bone loss in the rat model.
蜜蜂从各种植物来源采集的蜂胶是一种具有广泛生物活性的树脂状蜂巢产品。蜂胶已在饮食中广泛使用，以改善健康和预防疾病。本研究的目的是分析大鼠实验性牙周炎在全身给予蜂胶后的形态计量学和组织病理学变化。
40只Wistar大鼠分为四个实验组：未结扎组（NL；N = 10）；仅结扎组（LO；N = 10）；全身给予结扎和蜂胶组（每天100 mg/kg体重[Pro100；N = 10]或每天200 mg/kg体重[Pro200；N = 10]）。在下颌两个象限的下颌第一磨牙牙龈边缘放置丝线结扎。研究持续时间为11天，在此期间结束时处死动物。临床测量牙槽骨水平的变化，并对组织进行组织病理学检查，以评估研究组之间的差异。
11天结束时，LO组的牙槽骨丧失明显高于NL、Pro100和Pro200组（P <0.05）。LO组的破骨细胞数量明显高于NL、Pro100和Pro200组（P <0.05）。两种剂量的蜂胶均显著减少了与牙周炎相关的骨丧失，但两个蜂胶组之间的差异无统计学意义（P >0.05）。
本研究结果提供了形态学和组织学证据，表明全身给予蜂胶可预防大鼠模型中的牙槽骨丧失。
