Thomas Sarah E, Crozier Jayne, Catherine Aime M, Evans Harry C, Holmes Keith A
CABI Europe-UK, Silwood Park, Ascot, Berkshire, UK.
Mycol Res. 2008 Jul;112(Pt 7):852-60. doi: 10.1016/j.mycres.2008.01.008. Epub 2008 Feb 3.
Fungal endophytes were isolated from healthy stems and pods of Theobroma gileri, an alternative host of the frosty pod rot pathogen of cacao. Non-sporulating isolates were grouped into 46 different morphological species according to their colony morphology. Many of these morphospecies were assumed to be basidiomycetes and, therefore, were of particular interest. Basidiomycetous endophytes have received far less attention than ascomycetes and also have potential as biological control agents of the basidiomycetous pathogens of T. cacao: Moniliophthora roreri (frosty pod rot pathogen) and M. perniciosa (witches' broom disease). The morphospecies were further characterised by molecular analyses. Amplification of the nuLSU was undertaken for phylogenetic placement of these non-sporulating cultures and revealed a total of 31 different taxa of which 15 were basidiomycetes belonging to the class Agaricomycetes, and 16 ascomycetes primarily belonging to the Sordariomycetes.
从可可树霜霉病病原体的替代寄主吉列巧克力树（Theobroma gileri）的健康茎和豆荚中分离出了真菌内生菌。根据菌落形态，未产孢的分离物被归为46个不同的形态种。这些形态种中的许多被认为是担子菌，因此特别受关注。担子菌内生菌受到的关注远少于子囊菌，并且它们也有潜力作为可可树担子菌病原体的生物防治剂，这些病原体包括：可可树霜霉病菌（Moniliophthora roreri）和可可树女巫扫帚病菌（M. perniciosa）。通过分子分析对这些形态种进行了进一步表征。对核糖体大亚基（nuLSU）进行扩增，用于这些未产孢培养物的系统发育定位，结果显示共有31个不同的分类单元，其中15个是属于伞菌纲的担子菌，16个是主要属于粪壳菌纲的子囊菌。
