Rodrigues Eduardo B, Penha Fernando M, Furlani Bruno, Meyer Carsten H, Maia Mauricio, Farah Michel E
Vision Institute, Department of Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil.
Dev Ophthalmol. 2008;42:29-34. doi: 10.1159/000138911.
Lobeck and coworkers performed the first intravitreal application of vital dyes to visualize preretinal structures in 1932. Since then numerous investigators in the 20th century examined the use of biological stains through the endovenous, subretinal and intravitreal delivery route in order to identify vitreoretinal tissues and breaks. However, in the year 2000, a new surgical approach, recently coined chromovitrectomy, has arisen, which consists in the intraoperative application of vital dyes during vitrectomy. Initially fluorescein, and more recently indocyanine green, trypan blue, bromophenol blue, triamcinolone acetonide and patent blue have been used for the staining of preretinal membranes and tissues. Currently, many vital stains are under evaluation in animals for future clinical application during chromovitrectomy such as indigo carmine or light green. In this paper, several historical considerations in regard to the application of vital dyes in chromovitrectomy are discussed.
1932年，洛贝克及其同事首次将活性染料玻璃体内注射应用于视网膜前结构的可视化。从那时起，20世纪的众多研究人员通过静脉内、视网膜下和玻璃体内给药途径研究了生物染色剂的使用，以识别玻璃体视网膜组织和裂孔。然而，在2000年，一种新的手术方法——最近被称为染色玻璃体切除术出现了，它包括在玻璃体切除术中术中应用活性染料。最初使用荧光素，最近使用吲哚菁绿、台盼蓝、溴酚蓝、曲安奈德和专利蓝对视网膜前膜和组织进行染色。目前，许多活性染色剂正在动物身上进行评估，以便在染色玻璃体切除术中用于未来的临床应用，如靛胭脂或亮绿。本文讨论了关于活性染料在染色玻璃体切除术中应用的一些历史考量。
