Tohya Y, Taniguchi Y, Takahashi E, Utagawa E, Takeda N, Miyamura K, Yamazaki S, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Virology. 1991 Aug;183(2):810-4. doi: 10.1016/0042-6822(91)91016-a.
The nucleotide sequence of the 3'-end of the Japanese F4 strain of feline calicivirus (FCV) RNA was determined from a cloned cDNA of 3.5 kbp. We found three open reading frames (ORFs). The largest ORF encoded a 668-amino acid protein of 73,588 Da, which was presumably the capsid precursor protein of FCV and had significant amino acid sequence homology with the VP3 of picornaviruses. A small ORF at the extreme 3'-end was compared with that of the F9 strain of FCV, a vaccine strain originally from the U.S. Highly conserved amino acid sequences were shown, suggesting that this ORF might be functional and encode a putative 106-amino acid protein of 12,153 Da. The other ORF in the 5'-flanking region of the cDNA had consensus amino acid sequences conserved among the RNA-dependent RNA polymerases.
从一个3.5kbp的克隆cDNA中确定了猫杯状病毒（FCV）日本F4株RNA 3'-末端的核苷酸序列。我们发现了三个开放阅读框（ORF）。最大的ORF编码一个73588Da的668个氨基酸的蛋白质，推测它是FCV的衣壳前体蛋白，并且与微小核糖核酸病毒的VP3具有显著的氨基酸序列同源性。在最末端3'-端的一个小ORF与最初来自美国的疫苗株FCV F9株的小ORF进行了比较。结果显示出高度保守的氨基酸序列，表明这个ORF可能具有功能，并编码一个推测的12153Da的106个氨基酸的蛋白质。cDNA 5'-侧翼区域的另一个ORF具有在RNA依赖性RNA聚合酶中保守的共有氨基酸序列。
