猫杯状病毒衣壳蛋白基因的鉴定与序列测定
Identification and sequence determination of the capsid protein gene of feline calicivirus.
作者信息
Carter M J, Milton I D, Turner P C, Meanger J, Bennett M, Gaskell R M
机构信息
Division of Virology, School of Pathological Sciences, New Medical School, Newcastle upon Tyne, U.K.
出版信息
Arch Virol. 1992;122(3-4):223-35. doi: 10.1007/BF01317185.
Abstract
We have determined 4380 bases of the sequence from a cDNA clone containing the 3' end of feline calicivirus strain F9. We find four candidate open reading frames of which three are complete and comprise 245, 317 and 2012 nucleotides. The fourth continues toward the 5' end. We have expressed the largest complete open reading frame in E. coli. Sera raised to this antigen react specifically with the capsid protein and its intracellular precursor molecule. N-terminal sequence analysis of purified, mature capsid protein confirms this assignment and has identified the position at which precursor is cleaved.
摘要
我们已确定了来自包含猫杯状病毒F9株3'末端的cDNA克隆的4380个碱基的序列。我们发现了四个候选开放阅读框，其中三个是完整的，分别由245、317和2012个核苷酸组成。第四个开放阅读框向5'末端延伸。我们已在大肠杆菌中表达了最大的完整开放阅读框。针对该抗原产生的血清与衣壳蛋白及其细胞内前体分子发生特异性反应。对纯化的成熟衣壳蛋白进行N端序列分析证实了这一归属，并确定了前体被切割的位置。
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