Ju Ai-Hua, Xun Ya-Shu, Su Xiu-Lan, Bai Wan-Fu, Zhang Hui-Wen, Zhou Xiu-Juan
The School of Pharmaceutical Sciences, Inner Mongolia Medical College, Huhhot 010059, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2008 Mar;28(3):645-7.
The present study was to detect and compare the content of bile acids in ox bile powder and goat gall powder of Mongolia medicine by UV. Cholic acid with sulphuric acid were heated and dehydrated, and they produced conjugated double bond. The conjugated bond showed the same absorption peak in the ultraviolet range. The method of ultraviolet spectrophotometry can be used to detect and compare the content of bile acids in ox bile powder and goat gall powder. The result showed that the linear range was 0.003 3-0.016 7 mg x mL(-1) (r = 0.999 7). The average recovery (n = 5) of standard addition method of ox bile powder and goat gall powder was 98.48% (RSD = 1.79%) and 96.46% (RSD = 2.50%) respectively. The result of determination of five different samples showed that the content of bile acids in ox bile powder and goat gall powder was 40.85%-43.03% and 30.88%-32.64% respectively. The RSD of the analysis of ox bile powder and goat ball powder was 2.40% and 2.92% respectively, the RSD of stationary test of ox bile powder and goat ball powder in eight hours was 0.55% and 0.59% respectively, and the RSD of reproducibility of the analysis of ox bile powder and goat ball powder was 2.11% and 2.68% respectively. The method was simple, accurate, fast and easy to generalize and apply in many fields. It can be used to control the quality of ox bile powder and goat gall powder.
本研究旨在通过紫外分光光度法检测并比较蒙药牛胆粉和羊胆粉中胆汁酸的含量。胆酸与硫酸加热脱水后会产生共轭双键,该共轭双键在紫外区呈现相同的吸收峰。紫外分光光度法可用于检测和比较牛胆粉和羊胆粉中胆汁酸的含量。结果表明,线性范围为0.003 3 - 0.016 7 mg·mL⁻¹(r = 0.999 7)。牛胆粉和羊胆粉标准加入法的平均回收率(n = 5)分别为98.48%(RSD = 1.79%)和96.46%(RSD = 2.50%)。五个不同样品的测定结果表明,牛胆粉和羊胆粉中胆汁酸的含量分别为40.85% - 43.03%和30.88% - 32.64%。牛胆粉和羊胆粉分析的RSD分别为2.40%和2.92%,牛胆粉和羊胆粉八小时稳定性试验的RSD分别为0.55%和0.59%,牛胆粉和羊胆粉分析重现性的RSD分别为2.11%和2.68%。该方法简便、准确、快速,易于推广应用于多个领域,可用于控制牛胆粉和羊胆粉的质量。