Koyuturk Meral, Yanardag Refiye, Bolkent Sehnaz, Tunali Sevim
Department of Histology and Embryology, Faculty of Medicine, Istanbul Science University, 34394-Esentepe, Istanbul, Turkey.
Toxicol Ind Health. 2007 Aug;23(7):393-401. doi: 10.1177/0748233707081907.
Cadmium (Cd), a widely distributed toxic trace metal, has been shown to accumulate in liver after long- and short-term exposure. Cd (2 mg/kg/day CdCl2) was intraperitoneally given to rats for eight days. Vitamin C (250 mg/kg/day) + vitamin E (250 mg/kg/day) + sodium selenate (0.25 mg/kg/day) were given to rats by oral means. The animals were treated by anti-oxidants one hour prior to treatment with Cd every day. The degenerative changes were observed in the groups given only Cd and anti-oxidants + Cd. Metallothionein (MT) immunoreactivity increased in cytoplasm of hepatocytes of the rats given Cd when compared with controls. In a number of cells with Cd and anti-oxidants treatment, immunoreactivity increase was more than in the group given Cd only and nuclear MT expression was also detected. Cell proliferation was assessed with proliferating cell nuclear antigen (PCNA) immunohistochemistry. PCNA expressions increased in all groups more than in the controls. Anti-oxidants treatment increased cell proliferation. In the animals administered with Cd, an increase in serum aspartate (AST) and alanine (ALT) aminotransferases, liver glutathione (GSH) and lipid peroxidation (LPO) levels were observed. On the other hand, in the rats treated with anti-oxidants and Cd, serum AST and ALT, liver glutathione and LPO levels decreased. As a result, these results suggest that combined anti-oxidants treatment might be useful in protection of liver against Cd toxicity.
镉（Cd）是一种广泛分布的有毒痕量金属，已表明长期和短期接触后会在肝脏中蓄积。给大鼠腹腔注射Cd（2毫克/千克/天CdCl₂），持续八天。通过口服方式给大鼠给予维生素C（250毫克/千克/天）+维生素E（250毫克/千克/天）+亚硒酸钠（0.25毫克/千克/天）。每天在给予Cd前一小时用抗氧化剂对动物进行处理。在仅给予Cd的组和抗氧化剂+Cd组中观察到了退行性变化。与对照组相比，给予Cd的大鼠肝细胞胞质中金属硫蛋白（MT）免疫反应性增加。在一些接受Cd和抗氧化剂处理的细胞中，免疫反应性增加比仅给予Cd的组更多，并且还检测到了核MT表达。用增殖细胞核抗原（PCNA）免疫组织化学评估细胞增殖。所有组中的PCNA表达均比对照组增加。抗氧化剂处理增加了细胞增殖。在给予Cd的动物中，观察到血清天冬氨酸（AST）和丙氨酸（ALT）转氨酶、肝脏谷胱甘肽（GSH）和脂质过氧化（LPO）水平升高。另一方面，在接受抗氧化剂和Cd处理的大鼠中，血清AST和ALT、肝脏谷胱甘肽和LPO水平降低。结果，这些结果表明联合抗氧化剂处理可能有助于保护肝脏免受Cd毒性。
