Department of Histology and Embryology, Faculty of Medicine, Kadir Has University, Vefa Bey S. N5 80810 Gayrettepe, Istanbul, Turkey.
Environ Toxicol Pharmacol. 2006 May;21(3):235-40. doi: 10.1016/j.etap.2005.08.006. Epub 2005 Oct 24.
Acute effects of cadmium (Cd) and combined antioxidants were evaluated in Sprague-Dawley rat testes. The rats were subdivided into four groups. Cadmium chloride (2mg/kgday) injected intraperitoneally during 8 days. Vitamin C (250mg/kgday), vitamin E (250mg/kgday) and sodium selenate (0.25mg/kgday) were pretreated by gavage in both of control and cadmium injected rats. Testis lipid peroxidation and glutathione levels were determined by spectrophotometrically. In Cd treated rats, lipid peroxidation levels were increased and glutathione levels were decreased and combined antioxidants treatment was effective in preventing of lipid peroxidation and normalizing glutathione. In Cd treated animals, the degenerative changes were observed, but not observed in the administrated rats with Cd and antioxidants under the light microscope. Proliferating cell nuclear antigen, metallothionein and caspase-3 activities were evaluated by immunohistochemically. Proliferation activity was not seen in the spermatogonial cells of cadmium treated testis. Treatment with antioxidants in cadmium administrated testis leads to pronounced increase in proliferation activity. Cytoplasmic caspase-3 activity was determined in the spermatogenic cells but not spermatogonia in treatment of antioxidants with Cd. In control and treated with antioxidants animals, metallothionein expressions were localized in the cells of seminiferous tubules, although the expression only was observed in the interstitial cells of cadmium treated rats. Results demonstrated beneficial effects of combined vitamin C, vitamin E and selenium treatment in Cd toxicity.
急性镉（Cd）和联合抗氧化剂的影响在 Sprague-Dawley 大鼠睾丸中进行了评估。将大鼠分为四组。腹腔内注射氯化镉（2mg/kg·天），连续 8 天。维生素 C（250mg/kg·天）、维生素 E（250mg/kg·天）和亚硒酸钠（0.25mg/kg·天）通过灌胃在对照组和镉注射大鼠中进行预处理。通过分光光度法测定睾丸脂质过氧化和谷胱甘肽水平。在 Cd 处理的大鼠中，脂质过氧化水平增加，谷胱甘肽水平降低，联合抗氧化剂治疗有效预防脂质过氧化和使谷胱甘肽正常化。在 Cd 处理的动物中，观察到退行性变化，但在给予 Cd 和抗氧化剂的大鼠中未在光镜下观察到。通过免疫组织化学评估增殖细胞核抗原、金属硫蛋白和半胱氨酸天冬氨酸蛋白酶-3 活性。在 Cd 处理的睾丸的精原细胞中未观察到增殖活性。在 Cd 处理的睾丸中给予抗氧化剂治疗导致增殖活性显著增加。细胞质半胱氨酸天冬氨酸蛋白酶-3 活性在精母细胞中确定，但在抗氧化剂处理的 Cd 中未在精原细胞中确定。在对照组和抗氧化剂处理的动物中，金属硫蛋白表达定位于生精小管的细胞中，尽管仅在 Cd 处理的大鼠的间质细胞中观察到表达。结果表明，联合维生素 C、维生素 E 和硒治疗在 Cd 毒性中具有有益的作用。
