Ortiz Patricia García, Hansen Steen Honoré, Shah Vinod P, Menné Torkil, Benfeldt Eva
Department of Dermatology, Gentofte Hospital, 2900 Hellerup, Denmark.
Contact Dermatitis. 2008 Jul;59(1):23-30. doi: 10.1111/j.1600-0536.2008.01348.x. Epub 2008 Jun 1.
Determination of drug penetration in diseased skin represents a challenge.
To compare dermal microdialysis and tape-strip sampling of drug penetration in normal skin and skin with irritant dermatitis.
The two methodologies were employed simultaneously in 16 healthy volunteers. Samples were collected in a study of the penetration of a metronidazole cream formulation (Flagyl 1%) applied to forearm skin in both areas with irritant dermatitis and normal skin. Barrier perturbation and the depth of microdialysis probes were quantified by non-invasive bioengineering methods.
Microdialysis showed a significant threefold increase in metronidazole penetration in skin with irritant dermatitis compared with unmodified skin. Conversely, the concentration of metronidazole in tape-strip samples was significantly decreased in irritant dermatitis.
The selection of sampling methodology should be based on the skin layer of interest as well as the integrity of the skin barrier. Whenever the dermal tissue is the target for topical treatment, microdialysis sampling should be the method of choice.
确定药物在患病皮肤中的渗透情况是一项挑战。
比较正常皮肤和刺激性皮炎皮肤中药物渗透的真皮微透析和胶带条取样方法。
在16名健康志愿者中同时采用这两种方法。在一项关于甲硝唑乳膏制剂（1% 灭滴灵）应用于前臂皮肤刺激性皮炎区域和正常皮肤区域的渗透研究中收集样本。通过非侵入性生物工程方法对屏障扰动和微透析探针的深度进行量化。
微透析显示，与未改变的皮肤相比，甲硝唑在刺激性皮炎皮肤中的渗透显著增加了三倍。相反，在刺激性皮炎中，胶带条样本中甲硝唑的浓度显著降低。
取样方法的选择应基于感兴趣的皮肤层以及皮肤屏障的完整性。只要真皮组织是局部治疗的目标，微透析取样就应是首选方法。
