Moretton Cédric, Crétier Gérard, Nigay Henri, Rocca Jean-Louis
Laboratoire des Sciences Analytiques, UMR CNRS 5180, Université Claude Bernard Lyon 1, Université de Lyon, Bâtiment CPE, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
J Chromatogr A. 2008 Jul 11;1198-1199:73-9. doi: 10.1016/j.chroma.2008.04.081. Epub 2008 May 14.
Heart-cutting two-dimensional liquid chromatography/UV detection methods were developed to determine 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (noted as THI) in Class III caramel colours. In the first dimension, the caramel sample diluted in water was fractionated either by reversed-phase chromatography on C18 silica packing or by ion-exchange chromatography using strong cationic exchanger (SCX) stationary phase. In each case, THI elution domain was recovered in a loop and transferred to a second column packed with porous graphitic carbon (PGC) for the selective separation of THI. From the accuracy profiles with acceptance limit fixed at 20% and beta-expectation tolerance interval fixed at 90%, the two methods, C18-PGC and SCX-PGC, were validated for THI/caramel ratios included between 5-50 and 10-50 ppm, respectively. Finally, the two methods were compared for the analysis of numerous caramel samples.
开发了中心切割二维液相色谱/紫外检测方法，用于测定Ⅲ类焦糖色素中的2-乙酰基-4-(1,2,3,4-四羟基丁基)咪唑（记为THI）。在第一维中，用水稀释的焦糖样品通过在C18硅胶填料上的反相色谱法或使用强阳离子交换剂（SCX）固定相的离子交换色谱法进行分离。在每种情况下，THI洗脱区域在定量环中回收，并转移到填充有多孔石墨化碳（PGC）的第二根色谱柱上，用于THI的选择性分离。从接受限固定为20%且β期望容忍区间固定为90%的准确度分布图来看，C18-PGC和SCX-PGC这两种方法分别针对THI/焦糖比例在5-50 ppm和10-50 ppm之间的情况进行了验证。最后，对这两种方法在分析大量焦糖样品时进行了比较。
