Ergunay Koray, Tezel Gaye Guler, Dogan Asiye Isin, Ozen Haluk, Sirin Gozde, Ozbay Mehmet, Karabulut Erdem, Ustacelebi Semsettin
Department of Clinical Microbiology, Hacettepe University, Ankara, Turkey.
Pathol Res Pract. 2008;204(9):649-53. doi: 10.1016/j.prp.2008.04.004. Epub 2008 Jun 9.
Human Parvovirus B19 has previously been implicated in the pathogenesis of testicular germ cell tumors, but this could not have been confirmed. This study was designed to investigate the testicular persistence of Parvovirus B19 and possible associations with germ cell tumors. Paraffin-embedded or fresh tissues from 36 germ cell tumors, 20 germ cell aplasias, 26 normal testicular tissues, 20 liver tissues, and 20 spleen tissues were evaluated by two different molecular assays: a nested PCR for Parvovirus B19 capsid genes and a commercial quantitative real-time PCR. Positive results were further confirmed by another commercial real-time PCR assay. Viral DNA was detected in 3 of 36 (8.3%) germ cell tumors, but not in other groups. Viral loads observed in all positive samples were less than 20 IU/reaction, suggesting very low levels of viral replication or latency. These results either directly or indirectly imply the involvement of Parvovirus B19 with testicular germ cell tumors. Viral persistence in normal testis, germ cell aplasia tissues, or hepatic/splenic tissues was not observed in this study.
人细小病毒B19此前被认为与睾丸生殖细胞肿瘤的发病机制有关，但这一点尚未得到证实。本研究旨在调查细小病毒B19在睾丸中的持续存在情况以及与生殖细胞肿瘤的可能关联。通过两种不同的分子检测方法对来自36例生殖细胞肿瘤、20例生殖细胞发育不全、26例正常睾丸组织、20例肝脏组织和20例脾脏组织的石蜡包埋或新鲜组织进行评估：一种用于细小病毒B19衣壳基因的巢式PCR和一种商业定量实时PCR。阳性结果通过另一种商业实时PCR检测进一步确认。在36例生殖细胞肿瘤中有3例（8.3%）检测到病毒DNA，但在其他组中未检测到。在所有阳性样本中观察到的病毒载量均低于20 IU/反应，表明病毒复制水平或潜伏水平非常低。这些结果直接或间接地表明细小病毒B19与睾丸生殖细胞肿瘤有关。在本研究中未观察到病毒在正常睾丸、生殖细胞发育不全组织或肝/脾组织中的持续存在。
