Gray A, Guillou L, Zufferey J, Rey F, Kurt A M, Jichlinski P, Leisinger H J, Benhattar J
Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
J Gen Virol. 1998 Mar;79 ( Pt 3):573-9. doi: 10.1099/0022-1317-79-3-573.
Germ cell tumours (GCT) of the testis are the most common malignant tumours occurring in young adults. In view of the young age of patients, the increasing incidence of GCT and the overexpression of wild-type p53 observed in a majority of tumours, the possibility of the involvement of a virus in the development of this cancer was considered. Testicular GCT were analysed for the presence of cytomegalovirus and Epstein-Barr virus (EBV), which are known to cause overexpression of wild-type p53 protein, and parvovirus B19. The testicular tissue of 39 patients with testicular GCT and 12 patients with healthy testicular tissues was tested for presence of viral DNA by PCR. Neither cytomegalovirus nor EBV DNAs were detected in the 39 tumours analysed, but parvovirus B19 DNA sequences were demonstrated in the testicular tissue of 85% (33/39 cases) of patients with GCT. The sera of 16 of the 39 patients with GCT were tested for the presence of parvovirus B19 IgM and IgG. B19-specific IgG was detected in the sera of 11 patients (69%). Only one case was positive for parvovirus B19 IgM, which was also shown to have B19 genome sequences in the serum by PCR, indicating that in a majority of cases an acute B19 infection can be excluded as being the source of the B19 DNA sequences in the testis. B19 DNA could not be detected in normal testicular tissue and thus parvovirus B19 could play a role, direct or indirect, in the development of testicular GCT or have tropism for the tumour cells.
睾丸生殖细胞肿瘤（GCT）是年轻成年人中最常见的恶性肿瘤。鉴于患者年龄较轻、GCT发病率不断上升以及在大多数肿瘤中观察到野生型p53的过表达，人们考虑了病毒参与这种癌症发生发展的可能性。对睾丸GCT进行了巨细胞病毒、已知可导致野生型p53蛋白过表达的爱泼斯坦-巴尔病毒（EBV）以及细小病毒B19的检测。通过聚合酶链反应（PCR）检测了39例睾丸GCT患者和12例健康睾丸组织患者的睾丸组织中病毒DNA的存在情况。在所分析的39个肿瘤中均未检测到巨细胞病毒和EBV DNA，但在85%（33/39例）的GCT患者睾丸组织中发现了细小病毒B19 DNA序列。对39例GCT患者中的16例血清进行了细小病毒B19 IgM和IgG检测。在11例患者（69%）的血清中检测到了B19特异性IgG。仅1例细小病毒B19 IgM呈阳性，通过PCR检测其血清中也含有B19基因组序列，这表明在大多数情况下可排除急性B19感染是睾丸中B19 DNA序列的来源。在正常睾丸组织中未检测到B19 DNA，因此细小病毒B19可能在睾丸GCT的发生发展中直接或间接发挥作用，或者对肿瘤细胞具有嗜性。
