Diss T C, Pan L X, Du M Q, Peng H Z, Kerr J R
Department of Histopathology, Royal Free and University College London Medical School, UK.
Mol Pathol. 1999 Dec;52(6):349-52. doi: 10.1136/mp.52.6.349.
Parvovirus B19 has been demonstrated in testes of patients with germ cell tumours but not in controls, raising the possibility that the virus has an aetiological role in these tumours. The aims of this study were to investigate the association of the virus with germ cell tumours and to localise the virus histologically.
DNA was extracted from paraffin wax embedded sections of testes from 10 seminomas, eight teratomas, two mixed seminoma/teratomas, and 10 testes showing benign histology. Polymerase chain reaction (PCR) amplification of three regions within the NS and VP1/2 genes was carried out in duplicate on all samples. One PCR positive case (seminoma/teratoma) was examined by microdissection of histologically defined tissue components followed by PCR amplification of parvoviral sequences. Samples from PCR positive patients were immunostained using a B19 specific monoclonal antibody.
Seven cases were PCR positive, these comprised two of 10 seminomas, one of two mixed tumours, none of eight teratomas, and four of 10 benign controls. PCR analysis of the material microdissected from the seminoma/teratoma showed the presence of the virus in regions of seminoma, teratoma, intratubular germ cell neoplasia, normal tubules, and connective tissue. All patient samples studied immunohistochemically were negative.
This confirms the presence of parvovirus B19 in a proportion of germ cell tumours; however, in one patient, the virus was widespread in the tissue components and not confined to tumour cells. In addition, the virus was present in control benign testes. These data suggest that B19 might not be of aetiological importance in germ cell tumours of testis.
已在生殖细胞肿瘤患者的睾丸中检测到B19细小病毒，而在对照组中未检测到，这增加了该病毒在这些肿瘤中具有病因学作用的可能性。本研究的目的是调查该病毒与生殖细胞肿瘤的关联，并从组织学上定位该病毒。
从10例精原细胞瘤、8例畸胎瘤、2例混合性精原细胞瘤/畸胎瘤以及10例显示良性组织学的睾丸的石蜡包埋切片中提取DNA。对所有样本进行NS和VP1/2基因内三个区域的聚合酶链反应（PCR）扩增，一式两份。对1例PCR阳性病例（精原细胞瘤/畸胎瘤）进行组织学定义的组织成分显微切割，随后对细小病毒序列进行PCR扩增。使用B19特异性单克隆抗体对PCR阳性患者的样本进行免疫染色。
7例PCR阳性，其中包括10例精原细胞瘤中的2例、2例混合性肿瘤中的1例、8例畸胎瘤中无、10例良性对照中的4例。对从精原细胞瘤/畸胎瘤中显微切割的材料进行PCR分析显示，在精原细胞瘤、畸胎瘤、管内生殖细胞肿瘤、正常小管和结缔组织区域存在该病毒。所有进行免疫组织化学研究的患者样本均为阴性。
这证实了一部分生殖细胞肿瘤中存在B19细小病毒；然而，在1例患者中，该病毒在组织成分中广泛存在，并不局限于肿瘤细胞。此外，该病毒也存在于对照良性睾丸中。这些数据表明，B19在睾丸生殖细胞肿瘤中可能不具有病因学重要性。
