O'Neill Jenna, Roujeinikova Anna
Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN, England.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jun 1;64(Pt 6):561-3. doi: 10.1107/S1744309108012219. Epub 2008 May 30.
MotB is an essential component of the proton motive force-driven bacterial flagellar motor. It binds to the stress-bearing layer of peptidoglycan in the periplasm, anchoring the MotA/MotB stator unit to the cell wall. Proton flow through the channel formed by the transmembrane helices of MotA and MotB generates the turning force (torque) applied to the rotor. Crystals of recombinant Helicobacter pylori MotB have been obtained by the sitting-drop vapour-diffusion method using ammonium sulfate as a precipitant. These crystals belong to space group P4(1)2(1)2 or its enantiomorph P4(3)2(1)2, with unit-cell parameters a = 75.2, b = 75.2, c = 124.7 A. The asymmetric unit appears to contain one subunit, corresponding to a packing density of 3.4 A(3) Da(-1). The crystals diffract X-rays to at least 1.8 A resolution on a synchrotron-radiation source.
MotB是质子动力驱动的细菌鞭毛马达的一个必需组件。它与周质中承受压力的肽聚糖层结合，将MotA/MotB定子单元锚定在细胞壁上。质子通过MotA和MotB跨膜螺旋形成的通道流动，产生施加到转子上的转动力（扭矩）。通过使用硫酸铵作为沉淀剂的坐滴气相扩散法获得了重组幽门螺杆菌MotB的晶体。这些晶体属于空间群P4(1)2(1)2或其对映体P4(3)2(1)2，晶胞参数为a = 75.2，b = 75.2，c = 124.7 Å。不对称单元似乎包含一个亚基，对应于3.4 Å(3) Da(-1)的堆积密度。这些晶体在同步辐射源上能将X射线衍射到至少1.8 Å的分辨率。
