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成熟生物膜对不同剂量的LN12联合阿莫西林和克拉霉素反应的转录组分析

Transcriptome Analysis of the Response of Mature Biofilm to Different Doses of LN12 with Amoxicillin and Clarithromycin.

作者信息

Jin Fang, Yang Hong

机构信息

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 201100, China.

出版信息

Antibiotics (Basel). 2022 Feb 17;11(2):262. doi: 10.3390/antibiotics11020262.

DOI:10.3390/antibiotics11020262
PMID:35203863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8868532/
Abstract

is a gastrointestinal pathogen with a high infection rate. Probiotics are clinically used as an adjuvant to improve the cure rate and reduce the side effects of antibiotic treatment for . This study is the first to explore the effects of a cell-free supernatant of high- or low-dose LN12 combined with amoxicillin (AMX) and clarithromycin (CLR) on 3192 biofilms in terms of the biofilm biomass, survival rates, biofilm structure, and transcriptome. The results showed that the combination of the CFS of high-dose LN12 with AMX and CLR had stronger effects on the biofilm biomass, survival rate, and structure of 3192 biofilms. 3192 biofilms may increase the expression of NADH-related genes and downregulate flagellar assembly and quorum sensing-related receptor genes to deal with the stronger stress effects of high-dose LN12 with AMX and CLR. In conclusion, the biofilm biomass, survival rate, structure, and transcriptome results showed that the combination of LN12 CFS with AMX and CLR had dose effects. We recommend that compared with low doses, high doses of LN12 combined with AMX and CLR may be more effective for biofilm than low doses.

摘要

是一种感染率很高的胃肠道病原体。益生菌在临床上用作辅助药物，以提高治愈率并减少针对……的抗生素治疗的副作用。本研究首次从生物膜生物量、存活率、生物膜结构和转录组方面探讨高剂量或低剂量LN12的无细胞上清液与阿莫西林（AMX）和克拉霉素（CLR）联合使用对3192生物膜的影响。结果表明，高剂量LN12的无细胞上清液与AMX和CLR联合使用对3192生物膜的生物量、存活率和结构有更强的影响。3192生物膜可能会增加NADH相关基因的表达，并下调鞭毛组装和群体感应相关受体基因，以应对高剂量LN12与AMX和CLR联合使用带来的更强应激效应。总之，生物膜生物量、存活率、结构和转录组结果表明，LN12无细胞上清液与AMX和CLR联合使用具有剂量效应。我们建议，与低剂量相比，高剂量的LN12与AMX和CLR联合使用对生物膜的效果可能比低剂量更有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/5b2f2a3de9b2/antibiotics-11-00262-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/66c048ce6f4a/antibiotics-11-00262-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/47993e60c74d/antibiotics-11-00262-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/a427f2932810/antibiotics-11-00262-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/28d0acfc32c6/antibiotics-11-00262-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/03a710fc4d36/antibiotics-11-00262-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/3f8de635cca7/antibiotics-11-00262-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/918a783eea23/antibiotics-11-00262-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/2e04bc506f30/antibiotics-11-00262-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/5b2f2a3de9b2/antibiotics-11-00262-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/66c048ce6f4a/antibiotics-11-00262-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/47993e60c74d/antibiotics-11-00262-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/a427f2932810/antibiotics-11-00262-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/28d0acfc32c6/antibiotics-11-00262-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/03a710fc4d36/antibiotics-11-00262-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/3f8de635cca7/antibiotics-11-00262-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/918a783eea23/antibiotics-11-00262-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/2e04bc506f30/antibiotics-11-00262-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a64/8868532/5b2f2a3de9b2/antibiotics-11-00262-g009.jpg

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