Bhatia Prateek, Dey Pranab, Uppal Radha, Shifa Ruth, Srinivasan Radhika, Nijhawan Raje
Department of Cytology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Acta Cytol. 2008 May-Jun;52(3):329-33. doi: 10.1159/000325516.
To compare cytomorphology preservation and immunohistochemistry results between conventional cell blocks (CCB) and cytoscrape cell blocks (SCB).
Fine needle aspiration (FNAC) was done in 17 consecutive cases. Air-dried smears for May-Grünwald-Giemsa stain and wet-fixed smear for hematoxylin-eosin (H-E) stain were prepared. Simultaneously another pass was made in each case for preparation of material for CCB. One of the H-E-stained smears was spared for SCB. SCB was compared with CCB for cell morphology. Immunostaining was performed both cell blocks, as well as on FNA smears in 8 cases. Results were evaluated for intensity of staining and percentage of cells showing positivity.
CCB and SCB sections showed adequate cellularity in all cases. Morphologic preservation was good in SCB sections. There was good architectural and nuclear preservation in all cases of SCB. Immunostaining results showed better and clear intensity of staining with little background in all cell block cases.
SCB is a valuable technique in cell blocks from stained FNA smears. The cytomorphologic details are equally good in SCB and CCB. Additional panels of immunostaining can be done on SCB for better diagnosis and classification, particularly in cases in which repeat FNA is not possible.
比较传统细胞块（CCB）和刮片细胞块（SCB）的细胞形态学保存情况及免疫组化结果。
对连续17例患者进行细针穿刺抽吸（FNAC）。制备用于May-Grünwald-Giemsa染色的空气干燥涂片和用于苏木精-伊红（H-E）染色的湿固定涂片。同时，对每例患者再进行一次穿刺以制备CCB材料。将一张H-E染色涂片留作制备SCB之用。比较SCB与CCB的细胞形态。对8例患者的两种细胞块以及FNA涂片进行免疫染色。评估染色强度及阳性细胞百分比。
所有病例的CCB和SCB切片均显示细胞量充足。SCB切片的形态保存良好。所有SCB病例的结构和细胞核保存良好。免疫染色结果显示，所有细胞块病例的染色强度更好、更清晰，背景较少。
SCB是一种从染色FNA涂片中制备细胞块的有价值技术。SCB和CCB的细胞形态学细节同样良好。可对SCB进行额外的免疫染色组，以实现更好的诊断和分类，特别是在无法进行重复FNA的病例中。
