Mandell C P, George J W
Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95616.
Am J Vet Res. 1991 May;52(5):728-33.
Iron status, as determined by hematologic values, serum iron concentration, total iron-binding capacity, and zinc protoporphyrin concentration, was determined in 2 groups of 6 nonpregnant monkeys. Monkeys of groups 1 and 2 had 10 and 5%, respectively, of their blood volume withdrawn per week for up to 10 weeks or until blood hemoglobin concentration was less than or equal to 10 g/dl. A third group of 6 monkeys served as controls. The majority (8/12) of the monkeys became anemic (hemoglobin concentration, less than or equal to 10 g/dl) after approximately 30 to 70% (mean, 49%) of their blood volume was removed. Anemia was accompanied by decrease in serum iron concentration and percentage of transferrin saturation. Microcytosis, hypochromasia, and increased zinc protoporphyrin concentration, all hematologic characteristics of iron deficiency, developed later. The calculated iron stores ranged from 1 to 133 mg, with mean value of 51 mg. Iron-depleted monkeys had mean calculated available iron store of 20.8 mg, whereas iron-replete monkeys had mean available iron store of 114.0 mg. Changes were not observed in monkeys of the control group during the study period. None of the baseline hematologic or biochemical analytes measured were good predictors of iron stores. The diet used at the research center did not provide sufficient iron to prevent iron deficiency in most of the monkeys from which a total amount of 30 to 70% of blood volume at 5 or 10%/week was withdrawn. Studies requiring that much blood may need to be modified to include iron supplementation, reduction of sample volume, or iron replacement after termination of projects.
通过血液学指标、血清铁浓度、总铁结合力和锌原卟啉浓度来确定铁状态，对两组各6只未怀孕的猴子进行了检测。第1组和第2组的猴子每周分别抽取其血容量的10%和5%，持续10周，或直至血红蛋白浓度小于或等于10 g/dl。第三组6只猴子作为对照。在抽取约30%至70%（平均49%）的血容量后，大多数（8/12）猴子出现贫血（血红蛋白浓度小于或等于10 g/dl）。贫血伴有血清铁浓度和转铁蛋白饱和度百分比降低。小细胞性、低色素性以及锌原卟啉浓度升高，这些缺铁的血液学特征随后出现。计算出的铁储备量在1至133 mg之间，平均值为51 mg。缺铁猴子的平均计算可用铁储备为20.8 mg，而铁充足的猴子平均可用铁储备为114.0 mg。在研究期间，对照组猴子未观察到变化。所测量的基线血液学或生化分析物均不能很好地预测铁储备。研究中心使用的饮食不能提供足够的铁，以防止大多数每周按5%或10%抽取30%至70%血容量的猴子出现缺铁。需要抽取大量血液的研究可能需要进行调整，包括补充铁、减少样本量或在项目结束后进行铁替代。
