相思豆毒蛋白使核糖体失活的条件。
Requirements for the inactivation of ribosomes by gelonin.
作者信息
Sperti S, Brigotti M, Zamboni M, Carnicelli D, Montanaro L
机构信息
Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.
出版信息
Biochem J. 1991 Jul 1;277 ( Pt 1)(Pt 1):281-4. doi: 10.1042/bj2770281.
Abstract
Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP and a high-Mr factor present in the rabbit reticulocyte-lysate post-ribosomal supernatant. The kinetic constants of the gelonin-catalysed release of adenine from A. salina ribosomes are Km = 4.35 microM and Kcat. = 0.1 min-1 in the absence of cofactors, and Km = 1.15 microM and Kcat. = 108 min-1 in their presence. The last two values are similar to those measured for ricin A chain in the absence of cofactors (Km = 2.02 microM and Kcat. = 317 min-1).
摘要
相思豆毒蛋白使卤虫和兔核糖体失活需要ATP以及兔网织红细胞裂解物核糖体后上清液中存在的一种高分子量因子。在没有辅因子的情况下，相思豆毒蛋白催化从卤虫核糖体释放腺嘌呤的动力学常数为Km = 4.35微摩尔，Kcat. = 0.1分钟-1；在有辅因子存在时，Km = 1.15微摩尔，Kcat. = 108分钟-1。最后这两个值与在没有辅因子的情况下对蓖麻毒蛋白A链测得的值（Km = 2.02微摩尔，Kcat. = 317分钟-1）相似。
相似文献
1
Requirements for the inactivation of ribosomes by gelonin.相思豆毒蛋白使核糖体失活的条件。
Biochem J. 1991 Jul 1;277 ( Pt 1)(Pt 1):281-4. doi: 10.1042/bj2770281.
2
High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin.用于测定蓖麻毒素和相思子毒素从核糖体释放的腺嘌呤的高压液相色谱法和荧光法。
Biochem J. 1989 May 1;259(3):639-43. doi: 10.1042/bj2590639.
3
Differential requirement of ATP and extra-ribosomal proteins for ribosome inactivation by eight RNA N-glycosidases.八种RNA N-糖苷酶使核糖体失活对ATP和核糖体外蛋白质的不同需求。
Biochem Biophys Res Commun. 1992 Jan 31;182(2):579-82. doi: 10.1016/0006-291x(92)91771-h.
4
RNA present in post-ribosomal supernatants makes ribosomes susceptible to inactivation by gelonin and alpha-sarcin.核糖体后上清液中存在的RNA使核糖体易于被相思豆毒素和α-肌动蛋白灭活。
Biochem Mol Biol Int. 1994 Mar;32(3):585-96.
5
Preparation, optimization and characterization of a polyphenylalanine synthetizing system from Agrobacterium tumefaciens.根癌农杆菌多聚苯丙氨酸合成系统的制备、优化及表征
Cell Mol Biol (Noisy-le-grand). 1993 Sep;39(6):575-81.
6
Inactivation of ribosomes by an inhibitor of protein synthesis from Salmonella enteritidis.肠炎沙门氏菌蛋白质合成抑制剂对核糖体的失活作用。
New Microbiol. 1993 Jan;16(1):79-22.
7
Differential ricin sensitivity of rat liver and wheat germ ribosomes in polyuridylic acid translation.大鼠肝脏核糖体和小麦胚芽核糖体在多聚尿苷酸翻译中的蓖麻毒素敏感性差异
Arch Biochem Biophys. 1977 Aug;182(2):690-5. doi: 10.1016/0003-9861(77)90550-1.
8
3'-immature tRNA(Trp) is required for ribosome inactivation by gelonin,a plant RNA N-glycosidase.3'-未成熟的色氨酸转运核糖核酸（tRNA(Trp)）是植物RNA N-糖苷酶gelonin使核糖体失活所必需的。
Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):249-53. doi: 10.1042/bj3100249.
9
Effect of alpha-sarcin and ribosome-inactivating proteins on the interaction of elongation factors with ribosomes.α-肌动蛋白和核糖体失活蛋白对延伸因子与核糖体相互作用的影响。
Biochem J. 1989 Feb 1;257(3):723-7. doi: 10.1042/bj2570723.
10
Sensitivity of ribosomes from Agrobacterium tumefaciens to the ribosome-inactivating protein crotin 2 depending on the translocational state.根癌土壤杆菌核糖体对核糖体失活蛋白巴豆毒蛋白2的敏感性取决于转位状态。
Cell Mol Biol (Noisy-le-grand). 1996 Mar;42(2):151-8.
引用本文的文献
1
Functional Assays for Measuring the Catalytic Activity of Ribosome Inactivating Proteins.用于测量核糖体失活蛋白催化活性的功能测定法。
Toxins (Basel). 2018 Jun 14;10(6):240. doi: 10.3390/toxins10060240.
2
A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes.一种用于评估纯化的人核糖体内在活性的重建无细胞测定法。
Nat Protoc. 2016 Jul;11(7):1309-25. doi: 10.1038/nprot.2016.072. Epub 2016 Jun 23.
3
Biodistribution, pharmacokinetics, and nuclear imaging studies of 111In-labeled rGel/BLyS fusion toxin in SCID mice bearing B cell lymphoma.111In 标记的 rGel/BLyS 融合毒素在荷 B 细胞淋巴瘤 SCID 小鼠中的生物分布、药代动力学和核医学成像研究。
Mol Imaging Biol. 2011 Aug;13(4):721-9. doi: 10.1007/s11307-010-0391-0.
4
Effects of osmolarity, ions and compatible osmolytes on cell-free protein synthesis.渗透压、离子及相容性渗透剂对无细胞蛋白质合成的影响。
Biochem J. 2003 Jan 15;369(Pt 2):369-74. doi: 10.1042/BJ20021056.
5
In vitro and in vivo studies of a VEGF121/rGelonin chimeric fusion toxin targeting the neovasculature of solid tumors.一种靶向实体瘤新生血管的VEGF121/rGelonin嵌合融合毒素的体外和体内研究
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7866-71. doi: 10.1073/pnas.122157899.
6
Wheat ribosome-inactivating proteins: seed and leaf forms with different specificities and cofactor requirements.小麦核糖体失活蛋白：具有不同特异性和辅因子需求的种子型和叶型
Planta. 1995;197(4):633-40. doi: 10.1007/BF00191571.
7
Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.核糖体失活蛋白对大肠杆菌和根癌土壤杆菌翻译系统的影响。
J Bacteriol. 1993 Oct;175(20):6721-4. doi: 10.1128/jb.175.20.6721-6724.1993.
8
Maize ribosome-inactivating protein (b-32). Homologs in related species, effects on maize ribosomes, and modulation of activity by pro-peptide deletions.玉米核糖体失活蛋白（b - 32）。相关物种中的同源物、对玉米核糖体的影响以及前肽缺失对活性的调节。
Plant Physiol. 1995 Apr;107(4):1323-32. doi: 10.1104/pp.107.4.1323.
9
3'-immature tRNA(Trp) is required for ribosome inactivation by gelonin,a plant RNA N-glycosidase.3'-未成熟的色氨酸转运核糖核酸（tRNA(Trp)）是植物RNA N-糖苷酶gelonin使核糖体失活所必需的。
Biochem J. 1995 Aug 15;310 ( Pt 1)(Pt 1):249-53. doi: 10.1042/bj3100249.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Synthesis of hemoglobin in a cell-free system. I. Properties of the complete system.无细胞体系中血红蛋白的合成。I. 完整体系的性质。
J Biol Chem. 1962 Mar;237:760-7.
3
4
Reversal of the inhibitory effects of the pokeweed antiviral protein upon protein synthesis.商陆抗病毒蛋白对蛋白质合成抑制作用的逆转。
Biochim Biophys Acta. 1981 Jan 29;652(1):160-7. doi: 10.1016/0005-2787(81)90219-7.
5
Factor requirements for the tritin inactivation of animal cell ribosomes.动物细胞核糖体三素失活的因子需求
Biochim Biophys Acta. 1981 Jun 26;654(1):57-66. doi: 10.1016/0005-2787(81)90136-2.
6
Requirements for antiribosomal activity of pokeweed antiviral protein.商陆抗病毒蛋白抗核糖体活性的要求。
Biochim Biophys Acta. 1983 May 20;740(1):19-28. doi: 10.1016/0167-4781(83)90116-1.
7
Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA.用于真核生物信使核糖核酸翻译的经核酸酶处理的兔网织红细胞裂解物的制备与应用
Methods Enzymol. 1983;96:50-74. doi: 10.1016/s0076-6879(83)96008-1.
8
Met-tRNA hydrolase from reticulocytes specific for Met-tRNA f Met on 40S ribosomal subunits.来自网织红细胞的甲硫氨酸-tRNA水解酶，对40S核糖体亚基上的甲硫氨酸-tRNA f Met具有特异性。
Arch Biochem Biophys. 1972 Sep;152(1):385-97. doi: 10.1016/0003-9861(72)90228-7.
9
Effect of development on the translation of messenger RNA in Artemia salina embryos.发育对卤虫胚胎中信使核糖核酸翻译的影响。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2693-7. doi: 10.1073/pnas.71.7.2693.
10
Deacylated transfer ribonucleic acid as a factor for peptide chain initiation in rabbit reticulocyte systems.去酰化转运核糖核酸作为兔网织红细胞系统中肽链起始的一个因子。
J Biol Chem. 1968 Dec 25;243(24):6343-52.
Zotero 插件