α-肌动蛋白和核糖体失活蛋白对延伸因子与核糖体相互作用的影响。

Effect of alpha-sarcin and ribosome-inactivating proteins on the interaction of elongation factors with ribosomes.

作者信息

Brigotti M, Rambelli F, Zamboni M, Montanaro L, Sperti S

机构信息

Dipartimento di Patologia sperimentale dell'Università di Bologna, Italy.

出版信息

Biochem J. 1989 Feb 1;257(3):723-7. doi: 10.1042/bj2570723.

DOI:10.1042/bj2570723

PMID:2930482

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1135648/

Abstract

alpha-Sarcin from Aspergillus giganteus and the ribosome-inactivating proteins (RIPs) from higher plants inactivate the 60 S ribosomal subunit. The former is an RNAase, whereas RIPs are N-glycosidases. The site of cleavage of RNA and that of N-glycosidic depurinization are at one nucleotide distance in 28 S rRNA [Endo & Tsurugi (1987) J. Biol. Chem. 262, 8128-8130]. The effect of alpha-sarcin and that of RIPs on the interaction of elongation factors with Artemia salina (brine shrimp) ribosomes have been investigated. alpha-Sarcin inhibits both the EF1 (elongation factor 1)-dependent binding of aminoacyl-tRNA and the GTP-dependent binding of EF2 (elongation factor 2) to ribosomes, whereas two of the RIPs tested, ricin from Ricinus communis (castor bean) and volkensin from Adenia volkensii (kilyambiti), inhibit only the latter reaction. EF2 protects ribosomes from inactivation by both alpha-sarcin and ricin. The EF1-binding site is affected only by alpha-sarcin. The sensitivity of this site to alpha-sarcin is increased by pretreatment of ribosomes with ricin. A. salina ribosomes were highly resistant to the third RIP tested, namely gelonin from Gelonium multiflorum. All four proteins tested have, however, a comparable activity on the rabbit reticulocyte-lysate system.

摘要

来自巨大曲霉的α-肌动蛋白和高等植物的核糖体失活蛋白（RIPs）可使60 S核糖体亚基失活。前者是一种RNA酶，而RIPs是N-糖苷酶。RNA的切割位点和N-糖苷脱嘌呤位点在28 S rRNA中相隔一个核苷酸距离[远藤和鹤木（1987年）《生物化学杂志》262，8128 - 8130]。已经研究了α-肌动蛋白和RIPs对卤虫核糖体与延伸因子相互作用的影响。α-肌动蛋白既抑制氨基酰-tRNA依赖于延伸因子1（EF1）的结合，也抑制延伸因子2（EF2）依赖于GTP的与核糖体的结合，而所测试的两种RIPs，即来自蓖麻的蓖麻毒素和来自腺叶藤属的伏肯辛，仅抑制后一种反应。EF2可保护核糖体不被α-肌动蛋白和蓖麻毒素失活。EF1结合位点仅受α-肌动蛋白影响。用蓖麻毒素预处理核糖体可增加该位点对α-肌动蛋白的敏感性。卤虫核糖体对所测试的第三种RIP，即来自多花巴豆的巴豆球蛋白具有高度抗性。然而，所测试的所有四种蛋白质在兔网织红细胞裂解物系统中具有相当的活性。

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本文引用的文献

Synthesis of hemoglobin in a cell-free system. I. Properties of the complete system.无细胞体系中血红蛋白的合成。I. 完整体系的性质。

J Biol Chem. 1962 Mar;237:760-7.

The purification of diphtheria toxin and the isolation of crystalline toxin-protein.白喉毒素的纯化及结晶毒素蛋白的分离。

Br J Exp Pathol. 1958 Apr;39(2):139-49.

Gelonin, a new inhibitor of protein synthesis, nontoxic to intact cells. Isolation, characterization, and preparation of cytotoxic complexes with concanavalin A.gelonin，一种新的蛋白质合成抑制剂，对完整细胞无毒。与伴刀豆球蛋白A的细胞毒性复合物的分离、表征及制备。

J Biol Chem. 1980 Jul 25;255(14):6947-53.

Inhibition of protein synthesis in vitro by proteins from the seeds of Momordica charantia (bitter pear melon).苦瓜种子蛋白对体外蛋白质合成的抑制作用。

Biochem J. 1980 Feb 15;186(2):443-52. doi: 10.1042/bj1860443.

The site of action of alpha-sarcin on eukaryotic ribosomes. The sequence at the alpha-sarcin cleavage site in 28 S ribosomal ribonucleic acid.α-肌动蛋白对真核生物核糖体的作用位点。28S核糖体核糖核酸中α-肌动蛋白切割位点的序列。

J Biol Chem. 1982 Aug 10;257(15):9054-60.

Location of 5.8 S rRNA contact sites in 28 S rRNA and the effect of alpha-sarcin on the association of 5.8 S rRNA with 28 S rRNA.5.8 S核糖体RNA在28 S核糖体RNA中的接触位点位置以及α-肌动蛋白对5.8 S核糖体RNA与28 S核糖体RNA结合的影响。

J Biol Chem. 1983 Jan 10;258(1):333-8.

The ribonuclease activity of the cytotoxin alpha-sarcin. The characteristics of the enzymatic activity of alpha-sarcin with ribosomes and ribonucleic acids as substrates.细胞毒素α-肌动蛋白的核糖核酸酶活性。以核糖体和核糖核酸为底物时α-肌动蛋白的酶活性特征。

J Biol Chem. 1983 Feb 25;258(4):2662-7.

Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用。核糖体作为毒素的作用靶点。

Biochem J. 1973 Nov;136(3):677-83. doi: 10.1042/bj1360677.

Effect of development on the translation of messenger RNA in Artemia salina embryos.发育对卤虫胚胎中信使核糖核酸翻译的影响。

Proc Natl Acad Sci U S A. 1974 Jul;71(7):2693-7. doi: 10.1073/pnas.71.7.2693.

Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用：60S核糖体亚基作为毒素的作用靶点。

Biochem J. 1973 Nov;136(3):813-5. doi: 10.1042/bj1360813.

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