Oliva Rafael, Martínez-Heredia Juan, Estanyol Josep Maria
Human Genetics Research Group, IDIBAPS, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
Syst Biol Reprod Med. 2008 Jan-Feb;54(1):23-36. doi: 10.1080/19396360701879595.
A first step in the characterization of cellular functions is the identification of the proteins involved. The spermatozoon is an accessible cell that is particularly suited for analysis and indeed it was one of the first cells from which proteins were identified. An important advance in the identification of the protein composition of the spermatozoa was accomplished in the past using electrophoresis separation methods and protein sequencing with the Edmman procedure. However the recent developments in mass spectrometry have boosted the potential for the identification and study of sperm proteins. Catalogs of thousands of spermatozoan proteins in human and in model species are becoming available setting up the basis for subsequent research, diagnostic applications and development of specific treatments. The present article reviews the available scientific publications dealing with the composition and function of the sperm cell using a mass spectrometry proteomic approach.
细胞功能表征的第一步是识别其中涉及的蛋白质。精子是一种易于获取的细胞,特别适合进行分析,实际上它是最早被鉴定出蛋白质的细胞之一。过去,通过电泳分离方法和采用埃德曼降解法的蛋白质测序技术,在精子蛋白质组成的鉴定方面取得了重要进展。然而,质谱技术的最新发展提升了精子蛋白质鉴定和研究的潜力。人类和模式生物中数以千计的精子蛋白质目录正逐渐问世,为后续研究、诊断应用和特定治疗方法的开发奠定了基础。本文使用质谱蛋白质组学方法综述了有关精子细胞组成和功能的现有科学出版物。