ARC Centre of Excellence in Biotechnology and Development, Discipline of Biological Sciences, School of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, Australia.
Expert Rev Proteomics. 2009 Dec;6(6):691-705. doi: 10.1586/epr.09.76.
Understanding the cellular mechanisms that regulate mammalian sperm function is strategically important for both the management of male infertility and the development of novel approaches to male contraception. The spermatozoon is a transcriptionally and translationally suppressed cell that is released from the testes in a functionally inert state. Functional activation occurs in the epididymis and female tract via mechanisms that are entirely dependent on post-translational modifications. Proteomics is, therefore, the ideal technology to investigate this cell type. Herein, we comment on the proteomic analyses that have been applied to mammalian spermatozoa, including some concerns relating to data interpretation. Three comprehensive liquid chromatography-mass spectrometry lists of human, mouse and rat spermatozoa are then compared, insights into the molecular regulation of sperm function discussed and future directions speculated upon.
了解调节哺乳动物精子功能的细胞机制对于男性不育症的管理和新型男性避孕方法的开发都具有重要的战略意义。精子是一种转录和翻译受到抑制的细胞，在功能上处于静止状态，从睾丸中释放出来。功能激活发生在附睾和女性生殖道中，其机制完全依赖于翻译后修饰。因此，蛋白质组学是研究这种细胞类型的理想技术。本文评论了应用于哺乳动物精子的蛋白质组学分析，包括与数据解释有关的一些问题。然后比较了三个全面的人类、小鼠和大鼠精子的液相色谱-质谱分析列表，讨论了精子功能的分子调控，并推测了未来的研究方向。
