Oliva Rafael, de Mateo Sara, Estanyol Josep Maria
Human Genetics Research Group, IDIBAPS, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
Proteomics. 2009 Feb;9(4):1004-17. doi: 10.1002/pmic.200800588.
The spermatozoon is an accessible cell which can be easily purified and therefore it is particularly well suited for proteomic analysis. It is also an extremely differentiated cell with very marked genetic, cellular, functional and chromatin changes as compared to other cells, and has profound implications for fertility, embryo development and heredity. The recent developments in MS have boosted the potential for identification and study of the sperm proteins. Catalogues of hundreds to thousands of spermatozoan proteins in human and in model species are becoming available setting up the basis for subsequent research, diagnostic applications and the development of specific treatments. The present article reviews the available scientific publications dealing with the composition and function of the sperm cell using an MS proteomic approach.
精子是一种易于获取的细胞，能够轻松纯化，因此特别适合进行蛋白质组学分析。与其他细胞相比，它也是一种高度分化的细胞，具有非常显著的基因、细胞、功能和染色质变化，并且对生育、胚胎发育和遗传具有深远影响。质谱技术（MS）的最新进展提升了鉴定和研究精子蛋白的潜力。人类和模式物种中数百到数千种精子蛋白的目录正在出现，为后续研究、诊断应用和特定治疗方法的开发奠定了基础。本文使用质谱蛋白质组学方法综述了有关精子细胞组成和功能的现有科学出版物。
