Pereira Christiane Soares, Amorim Simone Duarte, Santos André Felipe das Mercês, Reis Cristhiane Moura Falavina Dos, Theophilo Grace Nazareth Diogo, Rodrigues Dália Dos Prazeres
Laboratório de Referência Nacional de Cólera e Outras Enteroinfecções Bacterianas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ.
Rev Soc Bras Med Trop. 2008 Mar-Apr;41(2):179-82. doi: 10.1590/s0037-86822008000200009.
Aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. In the present investigation, 2,323 rectal swab samples from newborns hospitalized in Rio de Janeiro were evaluated with a view to isolating Aeromonas. The samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the Oswaldo Cruz Institute of the Oswaldo Cruz Foundation. The swabs were subjected to enrichment in alkaline peptonated water with the addition of 1% sodium chloride (NaCl) and alkaline peptonated water plus 3% NaCl (37 degrees C/18-24h) and were streaked onto agar that was selective for Pseudomonas-Aeromonas (GSP Agar). Fifty-six Aeromonas strains were isolated, distributed as follows: Aeromonas caviae (42.8%), Aeromonas media (25%), Aeromonas veronii biogroup sobria (10.7%), Aeromonas hydrophila (9%), Aeromonas veronii biogroup veronii (5.3%), Aeromonas sobria (1.8%), Aeromonas jandaei (1.8%), Aeromonas schubertii (1.8%) and Aeromonas sp (1.8%). Resistance to one or more antimicrobial drugs was observed in 26.8% of the strains. Considering the importance of Aeromonas, there is an urgent need to warn about this in relation to nosocomial infection control.
气单胞菌属被认为在人类食用受污染的水和食物后具有致病性。在本调查中，对里约热内卢住院新生儿的2323份直肠拭子样本进行了评估，以分离气单胞菌。样本被收集并送至奥斯瓦尔多·克鲁兹基金会奥斯瓦尔多·克鲁兹研究所的国家霍乱和其他细菌性肠道感染参考实验室。拭子在添加1%氯化钠（NaCl）的碱性蛋白胨水中以及添加3% NaCl的碱性蛋白胨水中（37℃/18 - 24小时）进行增菌培养，然后划线接种到对假单胞菌 - 气单胞菌有选择性的琼脂（GSP琼脂）上。共分离出56株气单胞菌菌株，分布如下：豚鼠气单胞菌（42.8%）、中间气单胞菌（25%）、维罗纳气单胞菌温和生物群（10.7%）、嗜水气单胞菌（9%）、维罗纳气单胞菌维罗纳生物群（5.3%）、温和气单胞菌（1.8%）、詹氏气单胞菌（1.8%）、舒伯特气单胞菌（1.8%）和气单胞菌属（1.8%）。在26.8%的菌株中观察到对一种或多种抗菌药物的耐药性。考虑到气单胞菌的重要性，迫切需要就医院感染控制方面对此发出警告。
