Chi Xiaoyuan, Zhang Xiaowen, Guan Xiangyu, Ding Ling, Li Youxun, Wang Mingqing, Lin Hanzhi, Qin Song
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, P R China.
J Microbiol. 2008 Apr;46(2):189-201. doi: 10.1007/s12275-007-0223-3. Epub 2008 Jun 11.
Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal delta 12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).
多不饱和脂肪酸（PUFAs）是婴幼儿和成人营养的重要组成部分，因为它们是细胞膜的结构成分。脂肪酸去饱和酶负责在需要氧气和还原当量的反应中，将双键插入预先形成的脂肪酸链中。在本研究中，根据保守的富含组氨酸基序和系统发育谱，对七种真核光合微藻的脂肪酸去饱和酶进行了全基因组表征。对这些基因组的分析为真核植物脂肪酸生物合成途径的起源和进化提供了见解。此外，分离出莱茵衣藻中与小球藻微粒体δ12去饱和酶相似度最高的候选酶，并通过在酵母（酿酒酵母）中的异源表达验证了其功能。
