Bolz Sylvia, Schuettauf Frank, Fries Julia E, Thaler Sebastian, Reichenbach Andreas, Pannicke Thomas
Department of Ophthalmology, Institute for Ophthalmic Research, University of Tübingen, Röntgenweg 11, 72076 Tübingen, Germany.
Graefes Arch Clin Exp Ophthalmol. 2008 Sep;246(9):1249-54. doi: 10.1007/s00417-008-0872-x. Epub 2008 Jun 11.
To investigate the membrane physiology of Müller glial cells from retinae of DBA/2J mice (which develop ocular hypertension) and of C57BL/6 control mice of different ages.
Retinae were obtained at the ages of 3, 6, and 12 months from DBA/2J mice and from C57BL/6 controls. Immunohistochemistry was performed using antibodies against glial fibrillary acidic protein (GFAP). Whole-cell membrane currents, membrane potentials and capacitances were recorded from freshly isolated Müller cells.
Strong immunostaining for GFAP was found in Müller cells of 12-month-old DBA/2J mice, whereas only astrocytes were immunopositive in C57BL/6 retinae. No significant alterations of membrane currents or potentials of Müller cells from DBA/2J mice as compared to controls were observed at any age; however, the membrane capacitance was increased in Müller cells from DBA/2J mice at the age of 6 months.
Although Müller cells of DBA/2J mice display some symptoms of reactive gliosis, the lack of significant alterations of the membrane physiology confirm previous data demonstrating that these cells undergo a nonproliferative gliosis.
研究DBA/2J小鼠（会发生高眼压）和不同年龄的C57BL/6对照小鼠视网膜中米勒神经胶质细胞的膜生理学。
从3、6和12月龄的DBA/2J小鼠以及C57BL/6对照小鼠获取视网膜。使用抗胶质纤维酸性蛋白（GFAP）抗体进行免疫组织化学。从新鲜分离的米勒细胞记录全细胞膜电流、膜电位和电容。
在12月龄DBA/2J小鼠的米勒细胞中发现了强烈的GFAP免疫染色，而在C57BL/6视网膜中只有星形胶质细胞呈免疫阳性。与对照组相比，在任何年龄均未观察到DBA/2J小鼠米勒细胞膜电流或电位的显著变化；然而，6月龄DBA/2J小鼠的米勒细胞膜电容增加。
尽管DBA/2J小鼠的米勒细胞表现出一些反应性胶质增生的症状，但膜生理学缺乏显著变化证实了先前的数据，表明这些细胞经历了非增殖性胶质增生。
