Abe Emuri, Duverneuil Charlotte, de la Grandmaison Geoffroy, Alvarez Jean-Claude
Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, AP-HP, et Université Versailles Saint Quentin, 104 Boulevard Raymond. Poincaré, 92380 Garches, France.
J Forensic Sci. 2008 Jul;53(4):997-1000. doi: 10.1111/j.1556-4029.2008.00774.x.
A 54-year-old man was found dead with a bottle containing a brownish fluid near him. A toxicological screening was carried out in blood, urine, and stomach content. Only dichlorvos (2,2 dichlorovinyl O-O dimethylphosphate or DDVP) was found. A simple and rapid method, using DDVP-D(6) as an internal standard, was developed for the determination of DDVP by gas chromatography/mass spectrometry (GC/MS). The method was linear from 1 to 10 mg/L. Intraday and interday precisions were all <15%. DDVP concentration in cardiac blood was approximately four times higher than in peripheral blood. A high concentration was found in the heart showing a cardiac tropism of DDVP, kidney and lung concentrations being much lower. No DDVP was found in liver. DDVP stomach content was 38 g. The amount presumed ingested was 82 g, c. 1000 mg/kg of body. The oral LD(50) for DDVP ranges between 20 and 1090 mg/kg in animals but is not known for humans.
一名54岁男子被发现死亡，其身旁有一个装有褐色液体的瓶子。对血液、尿液和胃内容物进行了毒理学筛查。仅检测到敌敌畏（2,2 - 二氯乙烯基O - O - 二甲基磷酸酯或DDVP）。建立了一种以DDVP - D(6)为内标，通过气相色谱/质谱联用（GC/MS）测定敌敌畏的简单快速方法。该方法在1至10 mg/L范围内呈线性。日内和日间精密度均<15%。心血中敌敌畏浓度约为外周血的四倍。在心脏中发现高浓度敌敌畏，表明敌敌畏具有心脏嗜性，肾脏和肺部浓度则低得多。肝脏中未检测到敌敌畏。胃内容物中敌敌畏含量为38 g。推测摄入的量为82 g，约为1000 mg/kg体重。敌敌畏对动物的经口半数致死量（LD(50)）在20至1090 mg/kg之间，但人类的该数值未知。
