Codoñer-Franch Pilar, Bataller Alberola Amalia, Domingo Camarasa José V, Escribano Moya María C, Valls Bellés Victoria
Department of Paediatrics, Obstetrics and Gynaecology, Faculty of Medicine and Odontology, University of Valencia, Avenida Blasco Ibáñez, 15, 46010, Valencia, Spain.
Eur J Pediatr. 2009 Mar;168(3):321-7. doi: 10.1007/s00431-008-0762-6. Epub 2008 Jun 12.
The aim of this work was to examine the effect of dietary lipid intakes on the biomarkers of red cell antioxidant status in hypercholesterolaemic children. The study population included 34 children (18 boys and 16 girls) with cholesterol levels > or =5.2 mmol/l and 16 normolipidaemic children (9 boys and 7 girls) between 6 and 12 years of age. The status of the erythrocyte antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) were estimated spectrophotometrically. Dietary intake was assessed by 24-h recall and seven-day records. The hypercholesterolaemic children showed a decreased activity of antioxidant enzymes in relation to the control group. There was a negative correlation between energy intake and the activity of antioxidant enzymes (SOD and CAT) and GSH levels. Cholesterol intake was inversely correlated with CAT and GPx activity and GSH levels. The intake of polyunsaturated fat was positively correlated with the GPx activity. A decrease in the fat content of the diet for 6 months was proposed and 15 children followed the diet strictly. The activities of antioxidant enzymes in these children were significantly higher after the low-fat diet; the greatest increment was noted in the activity of GPx (91% with respect to the initial values), SOD was increased by 44% and CAT by 70%. We conclude that the intake of dietary lipids can modulate the antioxidant defence system, and an excess of energy and cholesterol has a negative influence on the antioxidant enzymes.
这项研究的目的是检测饮食中脂质摄入量对高胆固醇血症儿童红细胞抗氧化状态生物标志物的影响。研究对象包括34名6至12岁的儿童（18名男孩和16名女孩），其胆固醇水平≥5.2 mmol/l，以及16名血脂正常的儿童（9名男孩和7名女孩）。采用分光光度法评估红细胞抗氧化剂超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和还原型谷胱甘肽（GSH）的状态。通过24小时回忆法和7天记录评估饮食摄入量。与对照组相比，高胆固醇血症儿童的抗氧化酶活性降低。能量摄入与抗氧化酶（SOD和CAT）活性及GSH水平呈负相关。胆固醇摄入量与CAT和GPx活性及GSH水平呈负相关。多不饱和脂肪的摄入量与GPx活性呈正相关。建议将饮食中的脂肪含量降低6个月，15名儿童严格遵循该饮食。低脂饮食后，这些儿童的抗氧化酶活性显著升高；GPx活性升高最为显著（相对于初始值增加91%），SOD增加44%，CAT增加70%。我们得出结论，饮食中脂质的摄入可以调节抗氧化防御系统，能量和胆固醇摄入过多会对抗氧化酶产生负面影响。
