离液盐用于从酵母核蛋白中分离核糖核酸和蛋白质的方法。

The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins.

作者信息

Damodaran S, Kinsella J E

机构信息

Institute of Food Science, Stocking Hall, Cornell University, Ithaca, New York 14853.

出版信息

Biotechnol Bioeng. 1983 Mar;25(3):761-70. doi: 10.1002/bit.260250312.

DOI:10.1002/bit.260250312

PMID:18548692

Abstract

The effect of chaotropic salts on the dissociation of ribonucleic acid from yeast nucleoprotein complex was studied. The effectiveness of various salts on the dissociation of ribonucleic acid followed the chaotropic series; i.e., Cl(3)CCOONa = NaClO(4) > NaBr > NaCl. Treatment of the nucleoprotein complex with 0.5M Cl(3)CCOONa or NaClO(4) resulted in RNA removal of about 80%, whereas NaCl and NaBr removed only about 10 and 25%, respectively. Based on the results presented, a simple and novel method for industrial-scale preparation of single-cell proteins with low levels of nucleic acid is proposed.

摘要

研究了离液盐对酵母核蛋白复合物中核糖核酸解离的影响。各种盐对核糖核酸解离的有效性遵循离液序列；即，三氯乙酸钠 = 高氯酸钠 > 溴化钠 > 氯化钠。用0.5M三氯乙酸钠或高氯酸钠处理核蛋白复合物导致约80%的RNA去除，而氯化钠和溴化钠分别仅去除约10%和25%。基于所呈现的结果，提出了一种简单且新颖的工业规模制备低核酸含量单细胞蛋白的方法。