离液盐对核蛋白复合物的解离作用。
Dissociation of nucleoprotein complexes by chaotropic salts.
作者信息
Damodaran S, Kinsella J E
出版信息
FEBS Lett. 1983 Jul 11;158(1):53-7. doi: 10.1016/0014-5793(83)80675-9.
PMID:6345201
Abstract
The effect of various anions in destabilizing yeast nucleoprotein complexes followed the order F- less than Cl- less than Br- less than ClO-4 congruent to Cl3CCOO-. Treatment of yeast nucleoproteins with 0.5 M NaClO4 resulted in removal of 80% of RNA. Based on the results, a simple method for effective separation of RNA from ribosomal particles is proposed and the mechanism of RNA dissociation by anions is also discussed.
摘要
各种阴离子对酵母核蛋白复合物的去稳定作用顺序为
F⁻ ＜ Cl⁻ ＜ Br⁻ ＜ ClO₄⁻ ≈ Cl₃CCOO⁻。用0.5 M高氯酸钠处理酵母核蛋白可使80%的RNA去除。基于这些结果，提出了一种从核糖体颗粒中有效分离RNA的简单方法，并讨论了阴离子使RNA解离的机制。
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