Rozman M, Cervantes F, Rozman C
Postgraduate School of Hematology, Farreras Valentí, Hospital Clinic, University of Barcelona, Spain.
Ann Hematol. 1991 Jun;62(6):221-4. doi: 10.1007/BF01729836.
To ascertain whether progression from the chronic to the accelerated and blastic phases of chronic myeloid leukemia (CML) is associated with the loss of the myeloid differentiation antigens of the neutrophil granulocytes (NG), we analyzed two monoclonal antibodies (PMN 31D8 and PMN 13F6) recognizing normal peripheral blood NG membrane antigens in 49 patients in different evolutive stages of CML. Since five patients were studied twice, a total of 54 studies were carried out. Fourteen patients were evaluated at diagnosis, 12 in the controlled chronic phase within 1 year from diagnosis, 14 in the advanced chronic phase (median evolution 3.25 years), and 14 in the accelerated (five cases) or blastic (nine cases) phases. Fourteen normal subjects served as a control group. At diagnosis, a significant decrease in the positivity for both antigens was observed with respect to controls, probably due to the circulation of incompletely mature NG. In the early chronic phase the values were within the normal range, whereas a significant decrease was registered in the advanced chronic phase and especially in the accelerated/blastic phase. A negative correlation between the NG positivity for both markers and the time elapsed from the moment of obtaining the initial control of the disease was found, suggesting that a progressive loss of the myeloid antigens of the NG occurs during the evolutive course of CML. These results seem to confirm the usefulness of the NG myeloid differentiation antigen study as an evolutive parameter in CML.
为了确定慢性髓系白血病（CML）从慢性期进展到加速期和急变期是否与中性粒细胞（NG）髓系分化抗原的丧失有关，我们分析了两种识别正常外周血NG膜抗原的单克隆抗体（PMN 31D8和PMN 13F6），研究对象为49例处于CML不同演变阶段的患者。由于5例患者进行了两次研究，因此共进行了54次研究。14例患者在诊断时接受评估，12例在诊断后1年内处于慢性稳定期，14例处于慢性进展期（中位病程3.25年），14例处于加速期（5例）或急变期（9例）。14名正常受试者作为对照组。诊断时，观察到两种抗原的阳性率相对于对照组显著降低，这可能是由于未完全成熟的NG循环所致。在慢性早期，各项值在正常范围内，而在慢性进展期，尤其是在加速期/急变期，出现了显著下降。发现两种标志物的NG阳性率与疾病首次获得控制后经过的时间呈负相关，这表明在CML的演变过程中，NG的髓系抗原逐渐丧失。这些结果似乎证实了NG髓系分化抗原研究作为CML演变参数的有用性。
