Goldenberg M S, Beekman A C
Central Research Department, CIBA-GEIGY Corporation, Ardsley, New York 10502.
Biomaterials. 1991 Apr;12(3):267-74. doi: 10.1016/0142-9612(91)90033-7.
Using an aqueous solvent of high methanol content, we have been able to extend the use of Coomassie blue R protein staining to contact lens-type acrylate hydrogels of 35-80% water content. Protein deposition on a wide range of hydrogels was compared after exposure to protein and there was good agreement between in vitro and in vivo studies to assess deposit resistance. Staining was sensitive down to a 2 micrograms lysozyme/cm2 zone extending from one polymer surface to the other, and linear with protein content up to 40 micrograms/cm2. The staining method permits unusual deposit morphologies to be easily visualized and is best used for qualitative or semiquantitative evaluation of protein deposition during the development of new polymeric materials. We propose a new classification system for protein deposition based on the degree of Coomassie blue R staining.
使用高甲醇含量的水性溶剂，我们已能够将考马斯亮蓝R蛋白染色法的应用扩展到含水量为35 - 80%的隐形眼镜型丙烯酸酯水凝胶。在暴露于蛋白质后，对多种水凝胶上的蛋白质沉积情况进行了比较，并且在评估抗沉积性的体外和体内研究之间达成了良好的一致性。染色对于从一个聚合物表面延伸到另一个表面的低至2微克溶菌酶/平方厘米的区域很敏感，并且与高达40微克/平方厘米的蛋白质含量呈线性关系。该染色方法能够轻松地观察到不同寻常的沉积形态，并且最适合用于在新型聚合物材料研发过程中对蛋白质沉积进行定性或半定量评估。我们基于考马斯亮蓝R染色程度提出了一种新的蛋白质沉积分类系统。
